A model of cross-bridge attachment to actin in the A*M*ATP state based on x-ray diffraction from permeabilized rabbit psoas muscle

Biophysical Journal
Jin GuLeepo C Yu

Abstract

A model of cross-bridges binding to actin in the weak binding A*M*ATP state is presented. The modeling was based on the x-ray diffraction patterns from the relaxed skinned rabbit psoas muscle fibers where ATP hydrolysis was inhibited by N-phenylmaleimide treatment (S. Xu, J. Gu, G. Melvin, L. C. Yu. 2002. Biophys. J. 82:2111-2122). Calculations included both the myosin filaments and the actin filaments of the muscle cells, and the binding to actin was assumed to be single headed. To achieve a good fit, considerable flexibility in the orientation of the myosin head and the position of the S1-S2 junction is necessary, such that the myosin head can bind to a nearby actin whereas the tail end was kept in the proximity of the helical track of the myosin filament. Hence, the best-fit model shows that the head binds to actin in a wide range of orientations, and the tail end deviates substantially from its lattice position in the radial direction (approximately 60 A). Surprisingly, the best fit model reveals that the detached head, whose location thus far has remained undetected, seems to be located close to the surface of the myosin filament. Another significant requirement of the best-fit model is that the binding site on actin is ne...Continue Reading

References

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Citations

May 29, 2004·Journal of Biomechanics·M SonciniF M Montevecchi
Feb 22, 2012·Nature Structural & Molecular Biology·Boglárka H VárkutiAndrás Málnási-Csizmadia
Sep 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Theresia KraftBernhard Brenner
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·N A Koubassova, A K Tsaturyan
Jul 20, 2010·Annual Review of Analytical Chemistry·Paul W Bohn
Jun 10, 2008·Biophysical Journal·Natalia A KoubassovaAndrey K Tsaturyan
Aug 25, 2015·Journal of Molecular Biology·Clayton SmithVitold E Galkin
Jun 17, 2009·Biophysical Journal·P R Shorten, J Sneyd
Jul 30, 2016·Computer Methods in Biomechanics and Biomedical Engineering·Youjiro TamuraMasami Saito
Dec 6, 2002·The Journal of Biological Chemistry·Samantha P HarrisJill Trewhella
Aug 9, 2011·Journal of Molecular Biology·Albina OrlovaJill Trewhella

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