PMID: 3480537Dec 1, 1987

A model of high-affinity antibody binding to type III group B Streptococcus capsular polysaccharide

Proceedings of the National Academy of Sciences of the United States of America
M R WesselsD L Kasper


We recently reported that the single repeating-unit pentasaccharide of type III group B Streptococcus (GBS) capsular polysaccharide is only weakly reactive with type III GBS antiserum. To further elucidate the relationship between antigen-chain length and antigenicity, tritiated oligosaccharides derived from type III capsular polysaccharide were used to generate detailed saturation binding curves with a fixed concentration of rabbit antiserum in a radioactive antigen-binding assay. A graded increase in affinity of antigen-antibody binding was seen as oligosaccharide size increased from 2.6 repeating units to 92 repeating units. These differences in affinity of antibody binding to oligosaccharides of different molecular size were confirmed by immunoprecipitation and competitive ELISA, two independent assays of antigen-antibody binding. Analysis of the saturation binding experiment indicated a difference of 300-fold in antibody-binding affinity for the largest versus the smallest tested oligosaccharides. Unexpectedly, the saturation binding values approached by the individual curves were inversely related to oligosaccharide chain length on a molar basis but equivalent on a weight basis. This observation is compatible with a model...Continue Reading


Jun 30, 1995·Journal of Chromatography. a·L C Paoletti, K D Johnson
Apr 23, 2005·Infection and Immunity·Michael J CieslewiczCraig E Rubens
Nov 1, 1990·The Journal of Clinical Investigation·M R WesselsH J Jennings
Nov 15, 1996·The Journal of Clinical Investigation·D L KasperC J Baker
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y WangD L Kasper
Jun 3, 2015·Glycobiology·Omid Haji-GhassemiStephen V Evans
Apr 27, 2016·Infection and Immunity·Guillaume Goyette-DesjardinsMariela Segura
Nov 14, 2008·Clinical and Vaccine Immunology : CVI·Shannon L Harris, Philip Fernsten
Sep 7, 2007·Clinical and Vaccine Immunology : CVI·Samuel L MooreF Michon
Mar 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Giuseppe StefanettiD L Kasper
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wiltrud M Kalka-MollD L Kasper
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wiltrud M Kalka-MollD L Kasper
Apr 26, 2017·Proceedings of the National Academy of Sciences of the United States of America·Filippo CarboniFrancesco Berti


Apr 1, 1978·The Journal of Clinical Investigation·C J BakerD L Kasper
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Craig E RubensD L Kasper

Related Concepts

Immunoglobulin Activity
Structure-Activity Relationship
Immune Sera
Polysaccharides, Bacterial
Antibody Avidity
Streptococcus agalactiae
Antigen-Antibody Reactions

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Related Papers

International Archives of Allergy and Applied Immunology
V LindénP Christensen
Reviews of Infectious Diseases
C J Baker, D L Kasper
Journal of Perinatology : Official Journal of the California Perinatal Association
Michael S SchimmelArthur I Eidelman
© 2020 Meta ULC. All rights reserved