A model of partial hepatectomy in mice

Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research
Mehrdad NikfarjamChristopher Christophi

Abstract

A versatile, simple, and reproducible model of hepatectomy is essential for the study of liver regeneration and its effects on various pathological processes. A murine model of liver resection and regeneration suitable for research is described. Male inbred CBA mice 6-8 wk old were used in all experiments. The contribution of the hepatic lobes to the total liver mass was determined by wet weight measurements. Resection of 37% (n = 10) and 70% (n = 10) liver volume was performed using hemostatic clips to ligate the hepatic lobe pedicles. Animals were recovered and subsequently killed 21 days postoperatively Liver mass was determined and compared to control animals (n = 17) to assess the completeness of liver regeneration. There were no operative deaths in animals undergoing either 37% or 70% hepatectomy. The procedures could be performed expediently, and animal recovery was complete. Liver mass (grams) assessed 21 days postoperatively [mean (SE)] in both the 37% resection, 1.76 g (0.07), and 70% resection, 1.56 g (0.05), groups was not significantly different from control animals, 1.64 g (0.07) (p =.265). Thus, partial hepatectomy can be performed safely and rapidly in mice using haemostatic clip ligation of hepatic lobes, with ...Continue Reading

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Citations

May 17, 2011·PloS One·Futoshi Yazama, Akihiro Tai
Sep 21, 2012·Canadian Journal of Surgery. Journal Canadien De Chirurgie·Roderich BönninghoffJörg Sturm
Jul 2, 2010·World Journal of Gastroenterology : WJG·Tomohide HoriShinji Uemoto
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Nov 1, 2012·The Journal of Surgical Research·Shir Lin KohChristopher Christophi
Jan 27, 2007·The Journal of Surgical Research·Nadia HarunChristopher Christophi
Nov 22, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Paulo N A MartinsPeter Neuhaus
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Dec 21, 2005·Surgery·Mehrdad NikfarjamChristopher Christophi
Apr 10, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·George Van BurenLee M Ellis
Apr 4, 2015·Laboratory Animals·Y A NevzorovaC Liedtke
Jul 28, 2017·International Journal of Molecular Sciences·Stefan Hajny, Christina Christoffersen
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Nov 23, 2018·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·Baifeng QianEduard Ryschich

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