May 31, 1979

A model of translational control involving mRNA-associated proteins in chick embryonic muscles

Molecular Biology Reports
S K JainS Sarkar

Abstract

Highly purified poly(A)-containing free and polysomal mRNP particles have been isolated by chromatography of subcellular fractions of chick embryonic muscles on oligo-dT-cellulose and elution with low salt buffer at 45 degrees. The free and polysomal mRNP represent two distinct classes of macromolecules, the free particles having a more complex nucleoprotein organization than the polysomal particles. Comparison of the protein moieties of three classes of poly(A)-containing cytoplasmic mRNP -- those released from nuclei after in vitro transcription and processing (transported mRNP), the free, and polysomal mRNP -- strongly suggests that the majority of the mRNA-associated proteins are exchanged in the cytoplasm during the various functional states of mRNA. A model of translational control involving the participation of mRNA-associated proteins in chick embryonic muscles and by analogy in other differentiated eukaryotic cells is proposed.

Mentioned in this Paper

Polyribosomes
Adenine Polynucleotides
Nucleoproteins
Protein Biosynthesis
Chick Embryo
Muscle
Poly(A) Tail
Metazoa
Ribonucleoproteins

About this Paper

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