PMID: 7540703Mar 1, 1995Paper

A modification of biotinylated dextran amine histochemistry for labeling the developing mammalian brain

Journal of Neuroscience Methods
S L Ding, A J Elberger

Abstract

Biotinylated dextran amine (BDA) has proven to be an excellent anterograde tracer in adult mammalian brains, having some advantages over other anterograde tracers such as Phaseolus vulgaris-leucoagglutinin (PHA-L) and biocytin. However, results are inferior when BDA is used in neonatal mammals. To improve the sensitivity and quality of BDA labeling in neonatal mammalian brains, the tetramethylbenzidine-sodium tungstate (TMB-ST) method for horseradish peroxidase (HRP) histochemistry was modified and used in BDA histochemistry. After BDA application to the visual cortex of neonatal rat and cat, contralateral and ipsilateral cortical and subcortical regions were examined for BDA-labeled exons and terminals. The modified BDA histochemistry produced corpus callosum (CC) axons in neonatal rat and cat that were heavily and continuously labeled. The distribution, trajectories, branching and termination of individual CC axons, and even possible axon-axon contracts, were clearly identified in exquisite detail, even at low magnification. The quality of BDA labeling in the ipsilateral lateral geniculate nucleus and superior colliculus was similar to that of the CC axonal labeling. These results indicate that the modified BDA histochemistry...Continue Reading

References

Mar 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M Mesulam, D L Rosene
Oct 1, 1992·Journal of Neuroscience Methods·H M Brandt, A V Apkarian
Jan 8, 1992·The Journal of Comparative Neurology·K L Plummer, M Behan
Mar 1, 1992·Journal of Neuroscience Methods·C L VeenmanM G Honig
Sep 1, 1985·The Journal of Comparative Neurology·J Olavarria, R C Van Sluyters
Jan 20, 1981·The Journal of Comparative Neurology·G O Ivy, H P Killackey
Dec 1, 1984·The Journal of Comparative Neurology·G M Innocenti, S Clarke
Oct 1, 1993·Journal of Neuroscience Methods·X JiangA Burkhalter
Oct 20, 1994·Behavioural Brain Research·B R Payne

❮ Previous
Next ❯

Citations

Jul 11, 2000·The Journal of Comparative Neurology·M A KingsburyB L Finlay
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·T E ConturoM E Raichle
Apr 30, 2015·The Journal of Comparative Neurology·Claudia Distler, Klaus-Peter Hoffmann
Sep 5, 2007·The European Journal of Neuroscience·M E LacruzG Alarcón

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.