A modified chromogenic assay for the measurement of very low levels of factor VIII activity (FVIII:C)

Haemophilia : the Official Journal of the World Federation of Hemophilia
R YatuvM Baru

Abstract

A precise and sensitive chromogenic assay for the measurement of very low levels of factor VIII (FVIII) in plasma has been developed. The assay is based on modifications of a commercially available chromogenic assay. The modifications include reduction of sample final dilution factor and prolongation of the development period. The modified assay allows accurate and precise measurement of FVIII in the range of 0.001-0.02 IU mL(-1). The detection limit is 0.0005 IU mL(-1) and the quantitation limit is 0.0015 IU mL(-1). This assay can be used in research and to study the clinical efficacy of low circulating levels of FVIII in haemophilia A patients.

References

Jan 3, 2001·Haemophilia : the Official Journal of the World Federation of Hemophilia·P Petrini
Jun 28, 2003·Haemophilia : the Official Journal of the World Federation of Hemophilia·J IngerslevB Sørensen
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·J H McIntoshT W Barrowcliffe
Oct 14, 2004·Haemophilia : the Official Journal of the World Federation of Hemophilia·T W Barrowcliffe

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Citations

Jun 25, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·B VerbruggenW Van Heerde
Mar 24, 2016·Journal of Thrombosis and Haemostasis : JTH·S KitchenM Maas Enriquez
May 29, 2018·Haemophilia : the Official Journal of the World Federation of Hemophilia·A E BowyerJ P Antovic
Feb 29, 2020·Research and Practice in Thrombosis and Haemostasis·Saman AghighiPratima Chowdary
Jul 10, 2019·Haemophilia : the Official Journal of the World Federation of Hemophilia·Debnath MajiSanjay P Ahuja

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