A modified CULTEX system for the direct exposure of bacteria to inhalable substances

Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie
M Aufderheide, U Mohr

Abstract

Increasing attention is being paid to the impact on human health of inhaled gaseous compounds and complex mixtures such as cigarette smoke. The evaluation of the genotoxicity of such materials is mostly based on experiments with model substances or mixtures and condensates in the standard Ames assay. Due to the methodological difficulties of testing air contaminants in their natural gaseous or aerosolised state, there are no generally accepted concepts and techniques for effective exposure of bacteria under such conditions. Therefore, we established a novel experimental approach using an exposure device based on the cell exposure system CULTEX. This allows us to investigate chemically and physically unchanged atmospheres like mainstream cigarette smoke by exposing bacteria of Salmonella typhimurium strains directly on the surface of culture media. The CULTEX exposure device can be connected to gas or aerosol generating systems. The introduction of this exposure device in the field of inhalation genotoxicology offers new test strategies for the in vitro evaluation of a wide range of inhalable substances in both laboratory and ambient situations. A patent was applied for this technical solution.

References

Aug 28, 2003·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Michaela AufderheideDetlef Ritter

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Citations

Jan 30, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Amara L HolderCatherine P Koshland
Mar 22, 2014·International Journal of Molecular Sciences·Eleonore Fröhlich, Sharareh Salar-Behzadi
Jun 6, 2008·Expert Opinion on Drug Delivery·Michael Bur, Claus-Michael Lehr
May 9, 2014·Expert Opinion on Drug Metabolism & Toxicology·Amanda J Hayes, Shahnaz Bakand
Feb 2, 2010·Journal of Pharmacological and Toxicological Methods·Shahnaz Bakand, Amanda Hayes
Nov 11, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Naomi TsukueToru Nakajima
Apr 9, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Per Gerde
May 16, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Michaela Aufderheide
Jun 9, 2007·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Michaela Aufderheide, Helga Gressmann
Aug 12, 2005·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Michaela Aufderheide
Dec 27, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xiang LiJianping Xie
Jan 21, 2012·Inhalation Toxicology·Shahnaz BakandFinance Dechsakulthorn
Aug 22, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Eleonore FröhlichEva Roblegg
Sep 25, 2012·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Hidenori NaraMichaela Aufderheide
Jul 16, 2013·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·David Thorne, Jason Adamson
Jun 18, 2016·International Journal of Molecular Sciences·Shahnaz Bakand, Amanda Hayes
Jul 30, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xiang Li
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Aug 27, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Daniel PrimavessyMarius Hittinger

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