PMID: 20657158Jul 27, 2010Paper

A modified culture medium increases blastocyst formation and the efficiency of human embryonic stem cell derivation from poor-quality embryos

The Journal of Reproduction and Development
Yong FanXiaofang Sun

Abstract

Human embryonic stem cells (HESCs) are defined as self-renewing cells that retain their ability to differentiate into all cell types of the body. They have enormous potential in medical applications and as a model for early human development. There is a need for derivation of new HESC lines to meet emerging requirements for their use in cell replacement therapies, disease modeling, and basic research. Here, we describe a modified culture medium containing human recombinant leukemia inhibitory factor and human basic fibroblast growth factor that significantly increases the number of human blastocysts formed and their quality, as well as the efficiency of HESC derivation from poor-quality embryos. Culturing poor-quality embryos in modified medium resulted in a two-fold increase in the blastocyst formation rate and a seven-fold increase over the derivation efficiency in conventional medium. We derived 15 HESC lines from poor-quality embryos cultured in modified culture medium and two HESC lines from quality embryos cultured in conventional culture medium. All cell lines shared typical human pluripotent stem cell features including similar morphology, normal karyotypes, expression of alkaline phosphatase, pluripotency genes, such a...Continue Reading

References

Jul 1, 1997·Human Reproduction Update·D K Gardner, M Lane
Nov 6, 1998·Science·J A ThomsonJ M Jones
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·K HardyJ Stark
Sep 12, 2003·Stem Cells·Maisam MitalipovaSteven Stice
Nov 11, 2003·Fertility and Sterility·Liza DawsonRuth R Faden
Mar 5, 2004·The New England Journal of Medicine·Chad A CowanDouglas A Melton
Aug 31, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Andrea Jurisicova, Beth M Acton
Nov 3, 2004·The Journal of Clinical Investigation·Donald W Landry, Howard A Zucker
Jan 27, 2005·Reproductive Biomedicine Online·Nick StrelchenkoYury Verlinsky
May 4, 2005·Fertility and Sterility·Olga GenbacevSusan J Fisher
Mar 1, 2006·Lancet·Mahendra S Rao, Jonathan M Auerbach
Mar 18, 2006·Stem Cells·Curt I Civin, Mahendra S Rao
Sep 23, 2006·Stem Cells·Xin ZhangMiodrag Stojkovic
Jan 29, 2008·Nature Biotechnology·Paul H LerouGeorge Q Daley
Apr 16, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·S J KimberD R Brison
May 3, 2008·Stem Cells·Neta LavonNissim Benvenisty
Jul 28, 2009·Human Reproduction·Mieke GeensHilde Van de Velde

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Citations

Mar 4, 2014·Reproductive Biomedicine Online·Marine PoulainMichael Grynberg
Feb 4, 2012·The Tohoku Journal of Experimental Medicine·Yumei LuoXiaofang Sun
Jun 12, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xinling RenHanwang Zhang
Apr 25, 2012·Journal of Assisted Reproduction and Genetics·Xinjie ChenXiaofang Sun
Jul 25, 2015·Journal of Applied Toxicology : JAT·Lili DuDunjin Chen
Jun 20, 2015·Human Reproduction Update·Margot Van der JeughtBjörn Heindryckx
May 23, 2012·Stem Cell Reviews and Reports·María Vicenta CamarasaDaniel Bachiller

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