PMID: 3772017Aug 1, 1986Paper

A modified ferrozine method for the measurement of enzyme-bound iron

Journal of Biochemical and Biophysical Methods
M D DavisS Milstien

Abstract

A general procedure for the determination of the iron content of enzymes by digestion with methanesulfonic acid to release protein-bound iron has been developed. This procedure replaces the tedious and potentially hazardous method of wet ashing with concentrated nitric-sulfuric-perchloric acids. The method has been used to determine the stoichiometry of iron for nanomole quantities of heme-iron proteins, iron-sulfur proteins, complex iron-sulfur proteins, as well as in phenylalanine hydroxylase, an enzyme with iron in an undetermined coordination.

Citations

Sep 4, 2009·Acta Neuropathologica·Arnulf H KoeppenDanhong Li
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C C Landry, R W Topham
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P RibeiroS Kaufman
Dec 1, 2006·The Cerebellum·Susan MichaelArnulf H Koeppen
Mar 19, 2014·Analytical Biochemistry·Thomas M Jeitner
Nov 28, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sebastian E FloresJacqueline I Keenan
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Nov 14, 2020·ACS Synthetic Biology·Bhanu P JagilinkiVikas Nanda

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