A modified fluorescein derivative with improved water-solubility for turn-on fluorescent determination of Hg2+ in aqueous and living cells

Talanta
Shanyi GuangHongyao Xu

Abstract

To improve the water-solubility of heavy-metal sensing materials, a modified fluorescein-based derivative, acryloyl fluorescein hydrazine (ACFH), was designed and developed by incorporating a non-hydrogen-bonding group into the conjugated molecule for weakening intermolecular hydrogen-bonding interactions. In neutral water environments, ACFH presented a fluorescence-enhancement performance at λmax=512nm in the presence of Hg2+, which could be visualized by naked-eyes. Under the optimized conditions, the linear range of Hg2+ detection was 1.0-100×10-9molL-1 with a correlation coefficient of 0.9992 and a detection limit of 0.86×10-9molL-1. The recognition mechanism was confirmed to be a stable and irreversible 1:1 five-member ring complex between ACFH and Hg2+ with a coordination constant of 3.36×109. ACFH would possess a potential application in detecting Hg2+ for biological assay with low cytotoxicity.

Citations

Jan 18, 2018·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Yi S GuangYao H Xu
Jul 18, 2019·The Analyst·Mahnaz D GholamiEmad L Izake
Jul 14, 2021·Journal of Fluorescence·Keerthana SAnitha Varghese
Oct 27, 2021·Analytical Methods : Advancing Methods and Applications·Lei HuZhengquan Yan

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