A Modified Injector and Sample Acquisition Protocol Can Improve Data Quality and Reduce Inter-Instrument Variability of the Helios Mass Cytometer

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Brian H LeeAdeeb H Rahman

Abstract

Mass cytometry is a powerful tool for high-dimensional single cell characterization. Since the introduction of the first commercial CyTOF mass cytometer by DVS Sciences in 2009, mass cytometry technology has matured and become more widely utilized, with sequential platform upgrades designed to address specific limitations and to expand the capabilities of the platform. Fluidigm's third-generation Helios mass cytometer introduced a number of upgrades over the previous CyTOF2. One of these new features is a modified narrow bore sample injector that generates smaller ion clouds, which is expected to improve sensitivity and throughput. However, following rigorous testing, we find that the narrow-bore sample injector may have unintended negative consequences on data quality and result in lower median and higher coefficients of variation in many antibody-associated signal intensities. We describe an alternative Helios acquisition protocol using a wider bore injector, which largely mitigates these data quality issues. We directly compare these two protocols in a multisite study of 10 Helios instruments across 7 institutions and show that the modified protocol improves data quality and reduces interinstrument variability. These finding...Continue Reading

References

Jan 18, 2011·International Immunopharmacology·Tim F GretenFirouzeh Korangy
Mar 21, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Rachel FinckSean C Bendall
Jan 13, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sofie Van GassenYvan Saeys
Nov 1, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hermi R SumatohEvan W Newell

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Citations

Aug 12, 2020·International Journal of Radiation Oncology, Biology, Physics·Umair MahmoodJonathan D Schoenfeld
Jun 19, 2021·Computational and Structural Biotechnology Journal·Paulina RybakowskaConcepción Marañón
Jul 13, 2021·Frontiers in Immunology·Susan WestfallGiulio M Pasinetti
Jul 17, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bita SahafAdeeb Rahman

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