A modified microbial adhesion to hydrocarbons assay to account for the presence of hydrocarbon droplets

Journal of Colloid and Interface Science
Caroline Warne ZouekiSubhasis Ghoshal

Abstract

The microbial adhesion to hydrocarbons (MATH) assay has been used widely to characterize microbial cell hydrophobicity and/or the extent of cell adhesion to hydrophobic liquids. The classical MATH assay involves spectrophotometric absorbance measurements of the initial and final cell concentrations in an aqueous cell suspension that has been contacted with a hydrocarbon liquid. In this study, microscopic examination of the aqueous cell suspension after contact with hexadecane or a hexadecane/toluene mixture revealed the presence of hydrocarbon droplets. The hydrocarbon droplets contributed to the absorbance values during spectrophotometric measurements and caused erroneous estimates of cell concentrations and extents of microbial adhesion. A modified MATH assay that avoids such artefacts is proposed here. In this modified assay, microscopic examination of the aqueous suspension and direct cell counts provides cell concentrations that are free of interference from hydrocarbon droplets. The presence of hydrocarbon droplets was noted in MATH assays performed with three bacterial strains, and two different hydrocarbons, at ionic strengths of 0.2 mM and 20 mM and pH 6. In these experiments, the formation of quasi-stable hydrocarbon ...Continue Reading

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Citations

Oct 4, 2011·Applied Microbiology and Biotechnology·Hassan AbbasnezhadJulia M Foght
Nov 29, 2013·Environmental Science & Technology·Bahareh AsadishadNathalie Tufenkji
Aug 3, 2014·Microbial Pathogenesis·Shakiba Darvish Alipour AstanehSeyed Latif Mousavi Gargari
May 11, 2010·Colloids and Surfaces. B, Biointerfaces·Caroline Warne ZouekiNathalie Tufenkji
Jul 3, 2015·Soft Matter·Liana VaccariKathleen J Stebe
Apr 4, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Kerwin K W WongNathalie Tufenkji
Jan 25, 2018·Journal of the Science of Food and Agriculture·Shiau-Wei ChenHan-Tsung Wang
Aug 4, 2020·Journal of Industrial Microbiology & Biotechnology·Jacinta C Conrad
Aug 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Salihu IbrahimSiti Aqlima Ahmad
Jan 11, 2020·Frontiers in Microbiology·Holly C MayBernard P Arulanandam
Oct 5, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Ryan B McLayJacinta C Conrad

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