PMID: 6163465Jan 29, 1981Paper

A modified nucleotide in the poly(A) tract of maize RNA

Biochimica Et Biophysica Acta
J L Nichols, L Welder

Abstract

poly(A)+ RNA was isolated from maize by affinity chromatography on columns of oligo(dT)-cellulose. A modified nucleotide ('X') was detected in ribonuclease T2 digests of the RNA as part of a resistant dinucleotide. The dinucleotide was detected by means of the polynucleotide kinase-mediated transfer of a radioactive phosphate atom from adenosine triphosphate to the 5'-OH position of the dinucleotide. Intact poly(A) tracts were released from poly(A)+ RNA by digestion with ribonuclease T1 and A in a high salt buffer and were isolated by oligo(dT)-cellulose chromatography. The poly(A) preparation was found to consist of a series of polyadenylate fragments which varied in chain length from approximately 17 to greater than 70. The modified nucleotide was shown to occupy an internal position in these poly(A) tracts.

References

Dec 25, 1975·Journal of Molecular Biology·S Cory, J M Adams
Jul 26, 1979·Biochimica Et Biophysica Acta·J L Nichols
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Jan 1, 1976·Progress in Nucleic Acid Research and Molecular Biology·G Brawerman
Aug 10, 1976·Biochemistry·J R Greenberg
Dec 1, 1976·Cell·A J Shatkin
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D SheinessJ E Darnell
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Nov 15, 1973·European Journal of Biochemistry·J Klootwijk, R J Planta
Jun 27, 1980·Biochimica Et Biophysica Acta·J L Nichols, L Welder
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M BinaR G Deeley

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Citations

Nov 1, 1989·Archives of Gerontology and Geriatrics·W E MüllerH C Schröder
May 25, 2015·Journal of Molecular Cell Biology·Rune OuglandElisabeth Larsen
Jun 7, 2015·Current Opinion in Plant Biology·Rupert G Fray, Gordon G Simpson
Jun 28, 2016·Wiley Interdisciplinary Reviews. RNA·Thomas Philipp Hoernes, Matthias David Erlacher
May 16, 1984·Biochemical and Biophysical Research Communications·G Ford, J L Nichols

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