A modified procedure for the preparation of UDP-beta-L-(U-14C)rhamnose

Preparative Biochemistry
J KamsteegG van Nigtevecht


This paper describes the synthesis of UDP-L-(U-14C)rhamnose from UDP-D-(U-14C)glucose and NADPH using an enzyme preparation of Nicotiana tabacum var. Xanthi. A procedure to separate UDP-l-rhamnose from the other compounds in the reaction mixture is described. Optimal separation was achieved in ethanol 95%-1 M ammonium acetate (pH 3.8) (7:3, v/v) at 30 degrees C.


Mar 20, 1967·Archives of Biochemistry and Biophysics·G A BARBER, M T Chang
Nov 28, 1967·Biochimica Et Biophysica Acta·M M Vozari Hampe, N S Gonzalez
Oct 23, 1961·Biochemical and Biophysical Research Communications·G A BARBER, E F NEUFELD
May 25, 1962·Biochemistry·G A BARBER
Jul 3, 1962·Biochemical and Biophysical Research Communications·G A BARBER
Nov 1, 1963·Archives of Biochemistry and Biophysics·G A BARBER
Jan 18, 1965·Biochemical and Biophysical Research Communications·H KAUSS

Related Concepts

Molecular Stereochemistry
Rhamnose, L-Isomer
Ethanol Measurement
Plants, Toxic
Uridine Diphosphate Sugars
Enzyme agent
Ammonium acetate

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