A modified swim-up method reduces polyspermy during in vitro fertilization of porcine oocytes

Animal Reproduction Science
Chi-Hun ParkChang-Kyu Lee

Abstract

The general method of porcine in vitro fertilization (IVF), involving the co-culture of both gametes in a medium drop, is thought to be the main reason for the high incidence of polyspermy. The aim of this study was to reduce the polyspermic fertilization of porcine embryos during IVF by the modified swim-up method, based on general sperm swim-up technique. Within this design, a 70 microm pore sized cell strainer was used to separate the sperm pellet placed at the bottom of a tube from the mature oocytes placed within the upper region. The separation of gametes using this permeable barrier was to ensure that only motile sperm gained access to the oocytes. It was found that the rate of polyspermy was significantly lowered for the sperm preparations from three boar breeds in modified swim-up method when compared with that of the general microdrop method (p<0.05). However, the penetration rates were found to be similar in both methods for two boar breeds. The average occurrence of blastocysts with more total cell number was higher in the modified swim-up method, while no significant difference in blastocyst rates between the two IVF methods was observed. The frequency of normal diploid embryos was also significantly higher in the ...Continue Reading

References

Nov 1, 1991·Journal of Reproduction and Fertility·W H WangK Okuda
Oct 1, 1994·Molecular Reproduction and Development·F PellestorC Humeau
Sep 1, 1993·Journal of Reproduction and Fertility·H Funahashi, B N Day
Mar 1, 1996·Biology of Reproduction·R Lefebvre, S S Suarez
Jun 17, 2000·Zygote : the Biology of Gametes and Early Embryos·M BureauM A Sirard
Mar 22, 2002·Biology of Reproduction·Kazuhiro KikuchiTakashi Nagai
Dec 5, 2002·Reproduction in Domestic Animals = Zuchthygiene·D LechniakD Stanislawski
Oct 2, 2003·Theriogenology·Tod C McCauleyBilly N Day

❮ Previous
Next ❯

Citations

Nov 28, 2013·Theriogenology·Christopher G Grupen
Jun 16, 2012·Animal Reproduction Science·Wei ZhangXu Zhou
Jan 27, 2015·Zygote : the Biology of Gametes and Early Embryos·Jong-Nam OhChang-Kyu Lee
Jul 16, 2010·Reproduction in Domestic Animals = Zuchthygiene·M A GilE A Martinez
Dec 6, 2016·International Journal of Molecular Sciences·Timothy P SheetsBhanu P Telugu
Sep 7, 2012·Reproduction, Fertility, and Development·Jae Yeon HwangChang-Kyu Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.