PMID: 7021573Jun 1, 1981Paper

A modified technique for the detection of hepatitis B virus-specific DNA polymerase

Journal of Virological Methods
C T FangR Y Dodd

Abstract

A modified and improved technique for the detection of hepatitis B virus-specific DNA polymerase activity is described. DNA polymerase is released from Dane particles by mixing samples with the detergent Nonidet P-40 and beta-mercaptoethanol. After incubation of pretreated samples with a reaction mixture containing tritiated thymidine-methyl-5'-triphosphate (3H-TTP), DNA is precipitated onto a trichloroacetic acid (TCA)-treated paper. Unincorporated 3H-TTP is then chromatographically eluted with a 5% TCA solution and precipitated counts are determined. A sample is considered positive for DNA polymerase if the incorporated counts are significantly higher than the counts of a group of negative control samples. The modifications include pretreatment of the paper with TCA, chromatographic elution of unincorporated 3H-TTP with TCA solution, prefiltration of the sample through bacteriological filters, and use of sound statistical methods for evaluation of data. These changes have led to a highly reproducible, reliable and sensitive technique. The coefficient of variation of negative control samples from various test runs was in the range of 2.7-8.5%. A linear relationship between incorporated counts and DNA polymerase concentration w...Continue Reading

References

Mar 1, 1976·The Journal of Infectious Diseases·J E MaynardT R Bender
Jun 1, 1977·The Journal of Infectious Diseases·S Z Hirschman, E Garfinkel
May 1, 1971·Virology·W S Robinson, H L Robinson
Sep 27, 1974·Nature·D W BradleyD H Krushak

❮ Previous
Next ❯

Citations

Aug 1, 1987·Journal of Virological Methods·A J Zuckerman
May 1, 1989·Antiviral Research·R A de ManF J ten Kate
Aug 1, 1992·Antiviral Research·L BerkR A Heijtink
Aug 14, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas EfferthManfred Marschall
Jan 26, 1999·Antisense & Nucleic Acid Drug Development·W Xin, J H Wang
Jan 1, 1989·Journal of Gastroenterology and Hepatology·M IrshadB N Tandon
Jul 1, 1986·Journal of Clinical Pathology·H M MilborrowY E Cossart
Jan 1, 1983·Journal of Medical Virology·H A FieldsD W Bradley
Jan 1, 1982·Journal of Medical Virology·N NathR Y Dodd
Jan 1, 1983·Journal of Medical Virology·K N TsiquayeR Williams
Jul 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K N TsiquayeA J Zuckerman
Jan 1, 1987·Journal of Medical Virology·R H LokeI M Murray-Lyon
Mar 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H L JanssenS W Schalm
May 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K N TsiquayeA J Zuckerman
Aug 1, 1990·Gastroenterologia Japonica·M IrshadB N Tandon
Apr 1, 1990·Gastroenterologia Japonica·B M GandhiB N Tandon
Mar 31, 1986·The Medical Journal of Australia·P S DiegutisG McCaughan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.