A modular, plasmin-sensitive, clickable poly(ethylene glycol)-heparin-laminin microsphere system for establishing growth factor gradients in nerve guidance conduits

Biomaterials
Jacob L RoamDonald L Elbert

Abstract

Peripheral nerve regeneration is a complex problem that, despite many advancements and innovations, still has sub-optimal outcomes. Compared to biologically derived acellular nerve grafts and autografts, completely synthetic nerve guidance conduits (NGC), which allow for precise engineering of their properties, are promising but still far from optimal. We have developed an almost entirely synthetic NGC that allows control of soluble growth factor delivery kinetics, cell-initiated degradability and cell attachment. We have focused on the spatial patterning of glial-cell derived human neurotrophic factor (GDNF), which promotes motor axon extension. The base scaffolds consisted of heparin-containing poly(ethylene glycol) (PEG) microspheres. The modular microsphere format greatly simplifies the formation of concentration gradients of reversibly bound GDNF. To facilitate axon extension, we engineered the microspheres with tunable plasmin degradability. 'Click' cross-linking chemistries were also added to allow scaffold formation without risk of covalently coupling the growth factor to the scaffold. Cell adhesion was promoted by covalently bound laminin. GDNF that was released from these microspheres was confirmed to retain its activ...Continue Reading

References

Nov 1, 1988·Plastic and Reconstructive Surgery·A L Dellon, S E Mackinnon
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·L LiL J Houenou
Apr 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·M P LutolfJ A Hubbell
Oct 7, 2003·Annual Review of Biomedical Engineering·Christine E Schmidt, Jennie Baier Leach
Jan 6, 2004·Journal of Biomedical Materials Research. Part a·Terri Adams Kapur, Molly S Shoichet
Feb 27, 2004·Journal of Biomedical Materials Research. Part a·D SeliktarJ A Hubbell
Mar 10, 2004·Biotechnology and Bioengineering·Alison B PrattJeffrey A Hubbell
Dec 18, 2004·Biomaterials·Solitaire A DeLongJennifer L West
Apr 11, 2006·Biomacromolecules·Bradley K WackerDonald L Elbert
Jun 15, 2007·Biomaterials·Martin EhrbarMatthias P Lutolf
Aug 7, 2007·Journal of Neuroscience Methods·Daniel A HunterSusan E Mackinnon
Aug 9, 2008·Chemical Communications : Chem Comm·Jeremiah A JohnsonNicholas J Turro
Dec 17, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xiaoqin WangDavid L Kaplan
Jun 23, 2009·Cancer Biotherapy & Radiopharmaceuticals·Kido Nwe, Martin W Brechbiel
Feb 9, 2010·Biomaterials·Jordan S MillerChristopher S Chen
Feb 27, 2010·Biomaterials·James J MoonJennifer L West
Mar 24, 2010·Chemical Society Reviews·Charles E HoyleChristopher N Bowman
Jul 1, 2010·Biotechnology and Bioengineering·Matthew D WoodShelly E Sakiyama-Elbert
Sep 16, 2010·Chemistry of Materials : a Publication of the American Chemical Society·Cole A DeforestKristi S Anseth
Jun 28, 2012·Journal of Neural Engineering·Katelyn E Swindle-ReillyRebecca Kuntz Willits
Sep 21, 2013·Macromolecular Chemistry and Physics·Peter K NguyenDonald L Elbert

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Citations

Jan 12, 2017·Journal of Biomedical Materials Research. Part a·Dandan ShiHua Liao
May 10, 2017·Advanced Healthcare Materials·Alexander S CaldwellKristi S Anseth
Jun 22, 2017·Annual Review of Biomedical Engineering·Vineet GuptaMichael S Detamore
Apr 23, 2020·Journal of Biomedical Materials Research. Part a·Elizabeth A MaysHarini G Sundararaghavan
Jan 12, 2019·Neural Regeneration Research·Zhi-Yong WangBao-Guo Jiang
Oct 10, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Min GeJun Yang
Apr 13, 2021·Advanced Functional Materials·Alexander S CaldwellKristi S Anseth
Jan 1, 2020·Nature Reviews. Materials·Andrew C DalyJason A Burdick
Apr 6, 2018·ACS Applied Materials & Interfaces·Lingli LiTimothy C Hughes
Nov 9, 2019·ACS Applied Bio Materials·Shujie HouKyung Jae Jeong
Oct 10, 2016·ACS Biomaterials Science & Engineering·Tapomoy BhattacharjeeThomas E Angelini
Nov 17, 2021·Neural Regeneration Research·Kamilla Faritovna IdrisovaRuslan Faridovich Masgutov

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