A molecular comparison of T lymphocyte populations infiltrating the liver and circulating in the blood of patients with chronic hepatitis B: evidence for antigen-driven selection of a public complementarity-determining region 3 (CDR3) motif

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
G SingG Cooksley

Abstract

Despite a large number of T cells infiltrating the liver of patients with chronic hepatitis B, little is known about their complexity or specificity. To characterize the composition of these T cells involved with the pathogenesis of chronic hepatitis B (CHB), we have studied the clonality of VbetaT cell receptor (TCR)-bearing populations in liver tissue by size spectratyping the complementarity-determining region (CDR3) lengths of TCR transcripts. We have also compared the CDR3 profiles of the lymphocytes infiltrating the liver with those circulating in the blood to see whether identical clonotypes may be detected that would indicate a virus-induced expansion in both compartments. Our studies show that in most of the patients examined, the T cell composition of liver infiltrating lymphocytes is highly restricted, with evidence of clonotypic expansions in 4 to 9 TCR Vbeta subfamilies. In contrast, the blood compartment contains an average of 1 to 3 expansions. This pattern is seen irrespective of the patient's viral load or degree of liver pathology. Although the TCR repertoire profiles between the 2 compartments are generally distinct, there is evidence of some T cell subsets being equally distributed between the blood and the ...Continue Reading

Citations

Apr 26, 2003·Journal of Hepatology·Michelina Nascimbeni, Barbara Rehermann
Feb 9, 2011·Immunology and Cell Biology·John J MilesDavid A Price
Feb 21, 2008·World Journal of Gastroenterology : WJG·Jing YouJun-Hua Huang
Jul 19, 2002·Clinics in Liver Disease·Stephen A Geller
Nov 9, 2016·Journal of Immunological Methods·Claudio FozzaMaurizio Longinotti
Feb 28, 2018·Cell Biology and Toxicology·Xiao Liu, Jinghua Wu
Sep 28, 2004·The Journal of General Virology·Pierre BoudinotAbdenour Benmansour
May 11, 2004·World Journal of Gastroenterology : WJG·Shi-Jun ZhangBi-Jun Huang
Sep 13, 2003·World Journal of Gastroenterology : WJG·Ke-Xia WangChao-Pin Li

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