A molecular epidemiologic survey of HIV in Uganda. HIV Variant Working Group

AIDS
M A RayfieldT J Dondero

Abstract

Previous data, based on a small sampling of convenience, reported subtypes A, B, C, D, and G in Uganda, but neither the extent nor the proportion of these subtypes could be evaluated. To establish correctly the prevalence and distribution of HIV-1 subtypes, we analysed viral clades in 739 HIV-1-seropositive specimens from different areas of Uganda. Blood specimens from 1100 patients were collected in five districts of Uganda. Within this collection, 929 HIV-1-seroreactive samples underwent analysis of viral DNA, and 739 were selected for further subtyping in env or pol regions. Using a combination of subtype A- and D-specific probes to C2-V3 region and DNA sequencing, HIV-1 env subtypes were determined in 594 specimens: 341 were of subtype A (57.4%), 250 of subtype D (42.1%), and three of subtype C (0.5%). Sixty-two samples showed reactivity with both probes, suggesting potential mixed infections, cross-reactivity to probes, or possibly other subtypes. Subsequent sequence analysis of 19 randomly selected specimens revealed subtypes A (n = 4), D (n = 12), and C (n = 3). Sequence analysis of the 27 samples chosen from the remaining 83 samples, which could be amplified only with viral gp41 or protease gene primers, classified them...Continue Reading

References

Jul 1, 1991·AIDS Research and Human Retroviruses·J D OramJ C Booth
Apr 1, 1988·AIDS·E DelaporteF Brun-Vezinet
May 1, 1995·AIDS Research and Human Retroviruses·P KaleebuJ Weber
Apr 29, 1995·Lancet·C KunanusontN L Young
Jun 4, 1994·Lancet·I Loussert-AjakaF Simon
Mar 1, 1994·Journal of Virology·L G GürtlerL Kaptue
Jul 1, 1993·Nucleic Acids Research·N LarsenC R Woese
Jan 17, 1996·JAMA : the Journal of the American Medical Association·D J HuJ W Curran
Jul 1, 1996·Emerging Infectious Diseases·M A RayfieldG Schochetman

❮ Previous
Next ❯

Citations

Feb 26, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Julian H ElliottNick Crofts
May 29, 2000·AIDS Research and Human Retroviruses·G Becker-PergolaS H Eshleman
May 29, 2000·AIDS Research and Human Retroviruses·R DowningR B Lal
May 3, 2000·AIDS Research and Human Retroviruses·P KaleebuJ Whitworth
Apr 20, 2000·AIDS Research and Human Retroviruses·P KaleebuJ Oram
Aug 24, 2000·AIDS Research and Human Retroviruses·G Becker-PergolaS H Eshleman
May 23, 2002·AIDS Research and Human Retroviruses·Susan H EshlemanRobert W Shafer
Dec 19, 2002·AIDS Research and Human Retroviruses·Matthew E HarrisFrancine E McCutchan
Aug 9, 2002·AIDS Research and Human Retroviruses·Alexandra M KlevytskaSusan H Eshleman
May 29, 2007·AIDS Research and Human Retroviruses·Joshua T HerbeckJames I Mullins
Feb 16, 2006·AIDS Research and Human Retroviruses·José Carlos Couto-FernandezMariza G Morgado
Jan 31, 2002·AIDS·I D TattJ P Clewley
Aug 16, 2001·AIDS·M HoelscherUNKNOWN UNAIDS Network for HIV Isolation and Characterization
Nov 20, 2002·Journal of Virology·Grace P McCormackJonathan P Clewley
Aug 1, 2012·PloS One·Edson Oliveira Delatorre, Gonzalo Bello
Dec 29, 1998·Emerging Infectious Diseases·D PieniazekW Heneine
Nov 15, 2000·Emerging Infectious Diseases·D J HuR Lal
Dec 18, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·M LukwiyaM Clerici
Jun 8, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·P J WeidleE Lackritz
Jun 12, 1999·Virology·K TriquesM Peeters
Oct 27, 2015·Malaria Journal·Donald J KrogstadYéya T Touré
May 18, 2006·Emerging Infectious Diseases·James M ColbornDonald J Krogstad

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.