A molecular key for the identification of blow flies in southeastern Nebraska

Journal of Forensic Sciences
Upeka SamarakoonJ E Foster

Abstract

Immature blow flies (Calliphoridae) are typically the first colonizers of cadavers. Identification of the early instars using traditional, morphology-based keys is difficult because of their small size, similarity, and simplicity in external morphology. Information derived from molecular genetic data would augment the accurate identification of immature flies. Nine species of blow flies commonly found in southeastern Nebraska were used to examine the utility of molecular-based keys. Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) were investigated with 10 common, inexpensive, restriction enzymes from an amplicon of approximately 1500 bp spanning the mitochondrial cytochrome oxidase I gene. A simple molecular taxonomic key, comprising RFLP from the restriction enzymes HinfI and DraI, enabled the differentiation of all species used. Further development of PCR-RFLP, including more extensive and intensive examination of blow flies, would benefit forensic laboratories in the accurate identification of evidence consisting of immature blow flies.

References

Jan 1, 1992·Annual Review of Entomology·E P Catts, M L Goff
May 1, 1997·Clinics in Dermatology·P J SnijdersJ M Walboomers
May 25, 1999·Journal of Medical Entomology·J D Wells, F A Sperling
Aug 28, 1999·Forensic Science International : Synergy·Y Malgorn, R Coquoz
Apr 12, 2001·Journal of Medical Entomology·C W HaydenD G Mead
Jul 18, 2001·Forensic Science International : Synergy·J D Wells, F A Sperling
Jul 18, 2001·Forensic Science International : Synergy·J Stevens, R Wall
Jul 18, 2001·Forensic Science International : Synergy·C P Campobasso, F Introna
Apr 12, 2003·Forensic Science International : Synergy·H SchroederK Pueschel
Dec 4, 2003·Medical and Veterinary Entomology·M L HarveyI R Dadour
Mar 3, 2004·Journal of Medical Entomology·Wei-Yun ChenShiuh-Feng Shiao
Mar 3, 2004·Die Naturwissenschaften·Jens AmendtRichard Zehner
Dec 6, 2005·International Journal of Legal Medicine·Jeffrey D Wells, Diana W Williams
Mar 22, 2007·Medical and Veterinary Entomology·L A NelsonM Dowton
Aug 10, 2007·Annual Review of Entomology·Jeffrey D Wells, Jamie R Stevens
Apr 16, 2010·Forensic Science International : Synergy·Siew Hwa TanZulqarnain Mohamed

❮ Previous
Next ❯

Citations

Dec 3, 2014·Genomics·Meganathan P RamakodiDavid A Ray
Nov 13, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Joan A ChristenJohn E Foster
Jun 8, 2019·Journal of Medical Entomology·M Denise GemmellaroJessica L Ware

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.