A Molecular Method to Discriminate between Mass-Reared Sterile and Wild Tsetse Flies during Eradication Programmes That Have a Sterile Insect Technique Component

PLoS Neglected Tropical Diseases
Soumaïla PagabeleguemSophie Ravel

Abstract

The Government of Senegal has embarked several years ago on a project that aims to eradicate Glossina palpalis gambiensis from the Niayes area. The removal of the animal trypanosomosis would allow the development more efficient livestock production systems. The project was implemented using an area-wide integrated pest management strategy including a sterile insect technique (SIT) component. The released sterile male flies originated from a colony from Burkina Faso. Monitoring the efficacy of the sterile male releases requires the discrimination between wild and sterile male G. p. gambiensis that are sampled in monitoring traps. Before being released, sterile male flies were marked with a fluorescent dye powder. The marking was however not infallible with some sterile flies only slightly marked or some wild flies contaminated with a few dye particles in the monitoring traps. Trapped flies can also be damaged due to predation by ants, making it difficult to discriminate between wild and sterile males using a fluorescence camera and / or a fluorescence microscope. We developed a molecular technique based on the determination of cytochrome oxidase haplotypes of G. p. gambiensis to discriminate between wild and sterile males. DNA w...Continue Reading

References

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Citations

Sep 30, 2016·Parasites & Vectors·Soumaïla PagabeleguemJérémy Bouyer
Apr 16, 2020·Parasites & Vectors·Nicole J CulbertJérémy Bouyer
Jan 23, 2019·World Journal of Microbiology & Biotechnology·Sen XieYongqi Shao
Dec 28, 2017·PLoS Neglected Tropical Diseases·Mireille Djimangali BassèneJérémy Bouyer
Oct 7, 2021·Critical Reviews in Biotechnology·Sandipan BanerjeeRaj Narayan Roy

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Methods Mentioned

BETA
PCR

Software Mentioned

CodonCode Aligner
PATTEC

Related Concepts

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