PMID: 9188648Jul 1, 1997Paper

A monoclonal antibody (12G5) directed against CXCR-4 inhibits infection with the dual-tropic human immunodeficiency virus type 1 isolate HIV-1(89.6) but not the T-tropic isolate HIV-1(HxB)

Journal of Virology
J M StrizkiF González-Scarano

Abstract

We used a monoclonal antibody (12G5) directed against an extracellular domain of CXCR-4 to investigate the role of this receptor in infection of immortalized lymphoid cell lines, peripheral blood mononuclear cells (PBMCs), and primary brain microglia with a dual-tropic strain of human immunodeficiency virus (HIV-1(89.6)) and a T-tropic strain (HIV-1(IIIB)). Addition of antibody 12G5 to cells prior to and during infection with HIV-1(89.6) inhibited p24 production 100- to 10,000-fold in CEMx174 and 174-CD4 cells and about 10-fold in PBMC cultures but had no activity against infection of either monocyte-derived macrophages or brain microglia. In contrast, 12G5 had little or no effect on infection of CEMx174 cells with HIV-1(IIIB) or HIV-1(HxB). To identify the region of the HIV-1(89.6) envelope that confers sensitivity to 12G5, we used chimeric molecular clones. Chimeras containing the V3 loop region of HIV-1(89.6) were inhibited by 12G5 to the same degree as wild-type HIV-1(89.6) whereas replication of those viruses containing the V3 loop of HIV-1(HxB) was not inhibited by the antibody. A similar pattern was seen in infections of a U87 glioblastoma line that coexpresses CD4 and CXCR-4. Antibody 12G5 was also able to block fusion ...Continue Reading

References


❮ Previous
Next ❯

Citations

Jan 14, 1998·Nature Medicine·G A DonzellaJ P Moore
Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sergey A TrushinAndrew D Badley
Jun 30, 2007·Journal of Internal Medicine·S PhogatG B Karlsson Hedestam
Jun 10, 2011·The FEBS Journal·Andy ChevignéSabrina Deroo
Sep 5, 2001·Journal of Virology·F BaribaudR W Doms
Dec 14, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A SavarinoU Dianzani
Nov 19, 2003·European Journal of Biochemistry·Jianhua SuiWayne A Marasco
Nov 19, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Tonie Cilliers, Lynn Morris
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sven JähnichenMartine J Smit
Jun 15, 2012·The European Journal of Neuroscience·Annabelle Reaux-Le GoazigoStéphane Melik Parsadaniantz
Jul 18, 2001·The Journal of General Virology·T Dragic
Aug 29, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·J C Martín, J C Bandrés
Sep 10, 2002·AIDS·Julio Martín-GarcíaFrancisco González-Scarano
Apr 22, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeremiah D HerediaErik Procko
Jul 22, 2014·Journal of the American Chemical Society·Tao LiuFeng Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.