A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM-1 is a ligand for CD11c/CD18

European Journal of Immunology
J BlackfordJ M Wilkinson

Abstract

The rabbit CD11c molecule has been characterized by use of new monoclonal antibody, mAb 3/22. Expression of the p150,95 integrin (CD11c/CD18) has been shown by flow cytometry and immunohistochemistry to be restricted to monocytes, macrophages, dendritic cells and a small population of lymphocytes in peripheral blood. No expression on neutrophils could be demonstrated. Incubation of the newly derived CD8+ T cell line, BJ/873, with mAb 3/22 causes homotypic aggregation, which has been shown to be cell surface event that is not dependent on intracellular signaling or on receptor cross-linking. Inhibition studies show that the ligands responsible for this aggregation are CD11c/CD18 and ICAM-1, both of which are expressed on BJ/873. One other rabbit T cell line, K34, that also expresses p150,95 and ICAM-1, shows a similar aggregation response when stimulated with 3/22. Cell lines that express p150,95 but not ICAM-1 do not aggregate. These observations suggest that ICAM-1 is a ligand for activated p150,95.

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Citations

Mar 26, 1999·European Journal of Immunology·T ChenS Kozlowski
Nov 11, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·James O Jensen
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Oct 17, 2013·The Journal of Biological Chemistry·Liisa M UotilaCarl G Gahmberg
Mar 12, 2020·Frontiers in Immunology·Marie-Laure GolinskiSébastien Calbo
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Oct 20, 1999·The American Journal of Medicine·S Adler, H R Brady

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