PMID: 7540413May 1, 1995Paper

A monoclonal antibody (B724) to von Willebrand factor recognizing an epitope within the A1 disulphide loop (Cys509-Cys695) discriminates between type 2A and type 2B von Willebrand disease

British Journal of Haematology
O ChristopheJ P Girma

Abstract

Monoclonal antibody (MoAb) B724 to von Willebrand factor (vWF) completely inhibits its interaction with heparin, sulphatides and botrocetin and consequently botrocetin-induced binding of vWF to platelets. MoAb B724 has no effect on the binding of vWF to collagen or to ristocetin-treated platelets nor on vWF-dependent platelet aggregation induced with ristocetin and asialo-vWF-mediated platelet aggregation. MoAb B724 preferentially recognizes a conformation of native vWF, in solution, or immobilized through a coated antibody. It exhibits a markedly lower affinity for vWF immobilized onto collagen or plastic surfaces. Using proteolytic fragments of vWF, B724 epitope was localized within the 512-673 sequence of the A1 disulphide loop of vWF, MoAb B724 was used as second antibody in a two-site ELISA to test a series of patients with type 1, 2A, 2B and 2N vWD or haemophilia A and recombinant wild type or mutated vWFs. Results were compared with those obtained by control ELISAs performed using polyclonal antibodies. Using MoAb B724, strikingly lower levels of vWFAg were observed in plasma from most patients with type 2B vWD, and in seven out of the eight rvWF mutated close to or within the A1 disulphide loop. Therefore MoAb B724, whi...Continue Reading

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Citations

Oct 18, 2001·Current Opinion in Hematology·J Hambleton
Dec 25, 2012·Microvascular Research·Simat Siti FatimahAbdul Rahman Hayati
Aug 31, 2004·Journal of Thrombosis and Haemostasis : JTH·H UlrichtsI Tornai
Oct 14, 2009·Journal of Veterinary Emergency and Critical Care·Lisa SmartFern Tablin
Dec 31, 1997·Pediatric Hematology and Oncology·P PerutelliP G Mori
Jan 4, 2008·Journal of Bioinformatics and Computational Biology·Catherine L WorthTom L Blundell
May 1, 1999·Haemophilia : the Official Journal of the World Federation of Hemophilia·J Ingerslev, T Gürsel
Jun 26, 1998·Progress in Lipid Research·I Ishizuka

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