A monoclonal antibody-based enzyme-linked immunosorbent assay for detection of ustiloxin A in rice false smut balls and rice samples

Food Chemistry
Xiaoxiang FuLigang Zhou

Abstract

Ustiloxin A, a cyclopeptide mycotoxin, was isolated from the pathogenic fungus Villosiclava virens that causes rice false smut, a worldwide devastating rice disease. A monoclonal antibody (mAb) 2D3G5 was generated with ustiloxin A-bovine serum albumin conjugate. A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) was then developed. It possessed a median inhibition concentration (IC50) of 13.8 ng/mL and a working range of 2.8-72 ng/mL. The mAb 2D3G5 recognized ustiloxin B with the cross-reactivity as 4%. The average recoveries of ustiloxin A from rice false smut balls and peeled rice samples ranged from 92% to 117% and from 92% to 107%, respectively. Comparison of the concentrations of ustiloxin A in rice false smut balls detected by both icELISA and high performance liquid chromatography-photodiode array detection indicated that the developed icELISA was suitable for detection of ustiloxin A in rice food and feed samples.

References

Apr 29, 1994·Biochemical Pharmacology·R F LudueńaS Iwasaki
Jul 17, 2001·Journal of Agricultural and Food Chemistry·P CliquetB M Goddeeris
May 2, 2006·Mycopathologia·Michael Z ZhengJohann Binder
Mar 6, 2009·The Journal of Veterinary Medical Science·Shigeru MiyazakiKazuhide Morimoto
Oct 31, 2012·International Journal of Molecular Sciences·Tijiang ShanLigang Zhou

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