A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis: cross-reactivity with Leishmania major and Trypanosoma cruzi

Glycobiology
E SuzukiA H Straus

Abstract

A mouse monoclonal antibody, MEST-1, was produced against Band 1 glycolipid antigen of Paracoccidioides brasiliensis. The glycan structure of Band 1 antigen was recently elucidated and the monosaccharides sequence was defined as: Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins. The reactivity of MEST-1 MAb was determined by solid-phase radioimmunoassay and high performance thin layer chromatography immunostaining. Selective oxidation of galactofuranose residues and inhibition assays with different methyl-glycosides, revealed that MAb MEST-1 is directed against the terminal residue of beta-D-galactofuranose of Band 1, a phosphoglyceroglycolipid antigen of P. brasiliensis. By indirect immunofluorescence, it was observed that the epitope recognized by MEST-1 is accessible to the antibody in yeast forms of this fungus. Reactivity of MEST-1 with parasites known to express galactofuranose containing glycoconjugates was also analyzed by indirect immunofluorescence. A positive fluorescence was observed with promastigotes of Leishmania major and epimastigotes of Trypanosoma cruzi. GIPL-1 was identified as the antigen recognized by MEST-1 in Leishmania major, indicating that the MAb MEST-1 recognizes terminal galactofuranose residue ...Continue Reading

Citations

Jul 11, 2001·FEMS Microbiology Letters·G L WallisJ F Peberdy
Oct 21, 2009·The Journal of Biological Chemistry·Jakob EngelFrançoise H Routier
Sep 24, 2011·Glycobiology·Boris TefsenFrançoise H Routier
Jun 17, 2008·Eukaryotic Cell·Philipp S SchmalhorstFrançoise H Routier
Nov 20, 2002·Infection and Immunity·Erika SuzukiAnita H Straus
Feb 21, 2009·Archives of Microbiology·Graciele VicciniDavid Alexander Mitchell
Nov 26, 2015·Microbes and Infection·Elis Araujo MoraisAlfredo Miranda de Goes
Apr 2, 2008·International Journal of Biological Macromolecules·Lucimara M C CordeiroMarcello Iacomini
Jan 16, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sesselja OmarsdottirElin Soffia Olafsdottir
Feb 25, 2005·FEMS Microbiology Letters·Lucimara M C CordeiroMarcello Iacomini
Nov 29, 2012·PloS One·Ariane DesselleFrançois Paris
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·W Colli, M J Alves
Oct 15, 2008·Revista Da Sociedade Brasileira De Medicina Tropical·Ana Paula FerreiraHenrique Couto Teixeira
Aug 9, 2001·Journal of Endodontics·M L ZuoloH K Takahashi
May 17, 2018·MBio·Caroline Mota FernandesMaurizio Del Poeta
Sep 17, 2002·Medical Mycology·F A G BotteonE N Itano
Apr 26, 2003·Revista Da Sociedade Brasileira De Medicina Tropical·Márcia Luzia Ferrarezi MalufTerezinha Inez Estivalet Svidzinski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.