A monoclonal antibody specific for Pseudomonas aeruginosa amidase

Hybridoma
C NovoPaul R Brown

Abstract

Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.

References

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Feb 1, 1970·Analytical Biochemistry·A Levitzki
Aug 1, 1994·Protein Science : a Publication of the Protein Society·P Bork, E V Koonin

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