PMID: 3756878Oct 1, 1986Paper

A monoclonal antibody specific for the Yc subunit of rat liver glutathione S-transferase B

Cancer Research
K MatsumotoG Litwack

Abstract

A monoclonal antibody against rat liver glutathione S-transferase B, which consists of subunits Ya (Mr 27,000) and Yc (Mr 29,500), was produced in a mouse hybridoma system. The monoclonal antibody, BE6Yc1, was of the immunoglobulin G1 class and specifically recognized glutathione S-transferase B showing no cross-reactivity against the dimeric form of Ya, ligandin, in enzyme-linked immunosorbent assay. A glutathione S-transferase B-BE6Yc1 monoclonal antibody complex was identified from the zymogram pattern on starch gel electrophoresis. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis of rat liver glutathione S-transferase B, followed by Western blotting with BE6Yc1 antibody, a single polypeptide with a molecular weight of 29,500, identical to the Yc subunit of rat liver glutathione S-transferase B, was identified. The antibody showed no cross-reactivity with the Ya subunit with a molecular weight of 27,000 of either glutathione S-transferase B or ligandin. These results show that there are at least two distinct epitopes in the Ya and the Yc subunit. The monoclonal antibody did not block the catalytic activity of glutathione S-transferase B toward cumene hydroperoxide and possibly also 1-chloro-2,4-dinitrobenzene. Qu...Continue Reading

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