PMID: 6968776Nov 1, 1980Paper

A monoclonal antibody (TA-1) reactive with human T lymphocytes and monocytes

The Journal of Immunology : Official Journal of the American Association of Immunologists
T W LeBien, J H Kersey

Abstract

Several monoclonal antibodies were produced against the human T lymphocyte leukemia cell line HSB-2 by fusing spleen cells from hyperimmune mice with NS-1 myeloma cells. One clone, designated TA-1, was initially characterized for reactivity with normal and leukemic human hematopoietic cells. Using indirect immunofluorescence, TA-1 was shown to bind to approximately 75% of peripheral blood lymphocytes. Enrichment for T and B lymphocytes followed by double fluorochrome labeling studies revealed that most if not all E+ T lymphocytes and approximately 10% of B lymphocytes were TA-1+. TA-1 also reacted with approximately 67% of thymocytes, mixed lymphocyte culture-activated T lymphocytes, the entire peripheral blood monocyte population, and approximately 13% of nucleated bone marrow cells. TA-1+ cells in bone marrow included both phagocytic and nonphagocytic cells. TA-1 did not bind to peripheral blood granulocytes, red blood cells, or platelets. Studies of acute lymphoblastic leukemia (ALL) revealed that 6 of 11 T-ALL and 1 of 21 E-, SIg--ALL were TA-1+, whereas cells from 10 patients with chronic lymphocytic leukemia were TA-1-. Interestingly, TA-1 was able to distinguish acute myelomonocytic leukemic cells (TA-1+) from acute myel...Continue Reading

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.