A monoclonal antibody to feruloylated-(1-->4)-beta-D-galactan

Planta
Mads H ClausenJ Paul Knox

Abstract

We report the isolation and characterization of a monoclonal antibody, designated LM9, against feruloylated-(1-->4)-beta-D-galactan. This epitope is a structural feature of cell wall pectic polysaccharides of plants belonging to the family Amaranthaceae (including the Chenopodiaceae). Immuno-assays and immunofluorescence microscopy indicated that LM9 binding is specific to samples and cell walls obtained from species belonging to this family. In a series of competitive-inhibition enzyme-linked immunosorbent assays with potential oligosaccharide haptens, the most effective inhibitor was O-[6-O-(trans-feruloyl)-beta-D-galactopyranosyl]-(1-->4)-D-galactopyranose (Gal2F). LM9 is therefore a useful antibody probe for the analysis of phenolic substitution of cell wall pectic polymers and of cell wall structure in the Amaranthaceae including sugar beet (Beta vulgaris L.) and spinach (Spinacia oleracea L.).

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Citations

May 12, 2005·Planta·Florence GuilleminJean-François Thibault
Apr 7, 2010·Plant Physiology·Sivakumar PattathilMichael G Hahn
Feb 27, 2008·The Plant Journal : for Cell and Molecular Biology·Yeonkyeong LeeAnne Kathrine Hvoslef-Eide
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May 21, 2016·Frontiers in Plant Science·Peerapat RoongsatthamTimothy J Tranbarger
Jun 9, 2015·PloS One·Renê Gonçalves da Silva CarneiroRosy Mary dos Santos Isaias
Oct 7, 2004·Journal of Mass Spectrometry : JMS·Bernard Quéméner, Marie-Christine Ralet
Nov 19, 2015·Frontiers in Bioengineering and Biotechnology·Sivakumar PattathilMichael G Hahn
Sep 19, 2012·The Journal of Biological Chemistry·Henriette L PedersenWilliam G T Willats
Jul 14, 2019·Scientific Reports·Larissa StaackNinfa Rangel Pedersen
Mar 2, 2021·Food Research International·Dongyan ChenClare Kyomugasho

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