A monoclonal antibody to human angiogenin. Inhibition of ribonucleolytic and angiogenic activities and localization of the antigenic epitope

Biochemistry
J W FettS M Rybak

Abstract

A monoclonal antibody (mAb) to human angiogenin, a protein that induces formation of new blood vessels, was produced by somatic cell fusion techniques and designated as 26-2F. It is an IgGl kappa whose binding affinity, expressed as an IC50, is (1.6 +/- 0.1) x 10(-9) M as determined by a competition radioimmunoassay. mAb 26-2F neutralizes the ribonucleolytic activity of angiogenin as assessed by in vitro protein synthesis and tRNA degradation assays. It also effectively inhibits neovascularization induced by angiogenin on the chick chorioallantoic membrane. Epitope mapping indicates that the binding region of angiogenin recognized by mAb 26-2F is discontinuous and involves both Trp-89 and residues in the segment 38-41. This epitope is formed by two surface loops which are juxtaposed in the three-dimensional structure of human angiogenin recently determined by X-ray crystallography. Thus mAb 26-2F, along with similar antibodies under investigation, will facilitate structure/function studies of angiogenin, help define its physiological role, and lead to an understanding of the consequences of its inhibition in pathological situations in which angiogenin may be involved.

References

Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F Soncin
May 18, 1990·Cell·W G LaverS J Smith-Gill
Jul 1, 1991·The Journal of Bone and Joint Surgery. British Volume·T V King, B L Vallee
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T W HallahanB L Vallee
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G F HuB L Vallee
Jan 1, 1990·Annual Review of Biochemistry·D R DaviesS Sheriff
Sep 28, 1990·Biochemical and Biophysical Research Communications·M D Bond, B L Vallee
Jun 17, 1986·Biochemistry·R ShapiroB L Vallee
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R Bicknell, B L Vallee
Oct 15, 1989·Analytical Chemistry·D J StrydomJ F Riordan
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R Bicknell, B L Vallee
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J BadetD Barritault
Sep 24, 1985·Biochemistry·D J StrydomB L Vallee
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R ShapiroB L Vallee
Aug 1, 1988·Journal of Protein Chemistry·J W Harper, B L Vallee
Dec 5, 1988·FEBS Letters·P MaesA Tartar
Aug 23, 1988·Biochemistry·K KurachiB L Vallee
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D K St ClairB L Vallee
Aug 11, 1987·Biochemistry·R ShapiroB L Vallee
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G F HuB L Vallee
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·J Moroianu, J F Riordan
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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Citations

Mar 10, 1999·Biopolymers·M S Madhusudhan, S Vishveshwara
Apr 12, 2002·International Journal of Cancer. Journal International Du Cancer·Karen A OlsonJames W Fett
May 23, 2001·Drug Discovery Today·D J. HicklinP Bohlen
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·K A OlsonB L Vallee
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·W W Nagel, B L Vallee
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·K R AcharyaB L Vallee
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·V NobileR Shapiro
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·G F HuB L Vallee
Oct 19, 2010·BMC Evolutionary Biology·Francisco M CodoñerMario A Fares
Sep 22, 2007·BMC Evolutionary Biology·Daniel S OsorioMaria J Ramos
Jun 6, 2003·Bioscience, Biotechnology, and Biochemistry·Takao KosugeShoji Odani
May 21, 1999·Biochemical and Biophysical Research Communications·S J VerselisJ W Fett
Jul 27, 2001·Biochemical and Biophysical Research Communications·Z XuG Hu
Sep 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Norie YoshiokaGuo-fu Hu
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·R PiccoliJ W Fett
Sep 11, 2001·Biochemical and Biophysical Research Communications·S LiuG F Hu
Sep 12, 2006·Journal of Thrombosis and Haemostasis : JTH·A Tello-MontoliuG Y H Lip
Sep 22, 2007·Annals of Neurology·David WuGuo-Fu Hu
Aug 8, 2006·International Journal of Biological Macromolecules·Haiping Li, Shuo Wang
Nov 16, 2004·Biochemical and Biophysical Research Communications·Kalyan Sundar GhoshSwagata Dasgupta
Oct 22, 2013·Progress in Biophysics and Molecular Biology·Rajesh N Gacche, Rohan J Meshram
Sep 16, 1999·Biochemical and Biophysical Research Communications·X Y YeT B Ng

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