PMID: 8604015Mar 1, 1996Paper

A monoclonal antibody to human macrophage gangliosides inhibits macrophage migration

Journal of Leukocyte Biology
C S BerensonT F Murphy

Abstract

Gangliosides have diverse immunoregulatory properties. The gangliosides endogenous to macrophages may have immunoregulatory properties that distinguish them from other gangliosides. Gangliosides have been indirectly implicated in macrophage migration as putative cell surface receptors for migration inhibitory factor (MIF). In this study, a monoclonal antibody to human macrophage gangliosides (antibody 25F4) was developed and characterized. This is the first report of the development of monoclonal antibodies to gangliosides of macrophages of any species. Thin-layer chromatographic immunostaining indicated that antibody 25F4 recognized major gangliosides of human macrophages but did not recognized those previously identified as containing fucose. Immunofluorescent surface labeling of viable human macrophages indicated that antibody 25F4 recognized a surface-accessible epitope, present on all cells, and that this was abolished with lipid depletion of macrophage membranes. This epitope was not present on several human nonmacrophage cells. Finally, human macrophages pretreated with antibody 25F4 demonstrated striking inhibition of migration of an agarose droplet assay, whereas an irrelevant monoclonal antibody or monoclonal antibodi...Continue Reading

Citations

Feb 9, 2016·Immunological Investigations·Charles S BerensonSanjay Sethi
Nov 6, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·J FantiniN Yahi
Oct 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Eric J KochMargaret McFall-Ngai
Apr 5, 2002·Microbes and Infection·Hongqi LueJürgen Bernhagen

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