A monoclonal anticarbohydrate antibody detecting superfast myosin in the masseter muscle

Cell and Tissue Research
Svend Kirkeby

Abstract

Superfast-contracting muscle fibres (II M) were identified by ATPase staining and after incubation with an antiserum raised against myosin type II M and with an antibody raised against the Galalpha1-3Galbeta1-4GlcNAc structure. II M fibres were present in masseter muscles from cat, dog and Macaca fascicularis but not in limb muscles from the same animals and not in masseter muscles from rat, pig, cow or man. Electrophoresis and staining of blots from myosin preparations showed that the anticarbohydrate antibody detected myosin heavy chains from cat masseter but not myosin heavy chains from cat biceps. The alpha-galactose specific lectin Griffonia simplicifolia isolectin B4 (GS I B4) did not stain muscle fibres or myosin heavy chains. Therefore, the epitope on myosin heavy chains defined by the anticarbohydrate antibody is presumably not Galalpha1-3Galbeta1-4GlcNAc although the antibody staining was strongly inhibited after absorption by 10mM of this trisaccharide. Antibody staining of the muscle fibres was totally inhibited by adding 10mM p-nitrophenyl beta-D-glucuronide to the incubation medium. The results thus imply that an anticarbohydrate antibody distinctively detects a carbohydrate epitope specific for myosin in superfas...Continue Reading

Citations

Dec 17, 2003·Clinical Biochemistry·Svend KirkebyHans-Joachim Gabius
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