Abstract
A new method for the primary monolayer cultures of adult rabbit gastric mucous cells has been developed. Rabbit gastric mucosal cells were isolated with etylenediaminetetraacetic acid and collagenase. Cells were cultured in Coon's modified Ham's F-12 medium supplemented with 10% fetal bovine serum, 15mM HEPES buffer, antibiotics, and antimycotic. The cells reached confluency on days 3-4. Histochemically 92% of the cells contained PAS positive gramules (mucous cells), 3% of cells showed a strong reaction for succinic dehydrogenase activity (parietal cells), 2% of the cells showed positive granules by Bowie staining (chief cells), and G6PDH staining was positive in 5% of the cells (surface mucous cells). Fibroblasts were rarely seen until day 7 (less than 1%). Thus rabbit cultured gastric cells were considered to be mainly comosed of mucous neck cells. These cells produced prostaglandin (PG) E2 and PGI2. Quantitatively cultured cells synthesized 1.475 +/- 0.039 ng/mg protein/hour of PGE2 and 0.244 +/- 0.042 pg/mg protein/hour of PGI2. This relatively simple and convenient technique provides a useful model for the study of cellular functions of gastric mucosa.
References
Aug 1, 1976·Acta Physiologica Scandinavica·T BerglindhK J Obrink
Feb 1, 1978·The Journal of Clinical Investigation·A H Soll
Aug 1, 1986·Prostaglandins·H HiraishiT Sugimoto
Mar 1, 1987·Gastroenterology·A TeranoK J Ivey
Jun 1, 1987·Gastroenterologia Japonica·S OtaK J Ivey
Dec 1, 1988·Gut·S OtaK J Ivey
Nov 1, 1988·Prostaglandins·S OtaK J Ivey
Aug 1, 1988·Digestive Diseases and Sciences·S OtaK J Ivey
Apr 1, 1985·British Journal of Pharmacology·S PostiusI Szelenyi
Jan 1, 1984·Gut·A TeranoK J Ivey
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A AyalonA H Soll
Jul 1, 1983·Digestive Diseases and Sciences·A TeranoK J Ivey
Mar 1, 1982·In Vitro·C D LogsdonT E Machen
Aug 22, 1980·European Journal of Pharmacology·M L SkoglundA S Nies
Jul 1, 1957·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M NACHLASA M SELIGMAN
Citations
Nov 1, 1996·Journal of Gastroenterology and Hepatology·M TakahashiM Omata
Jul 1, 1994·Journal of Gastroenterology and Hepatology·S WatanabeN Sato
Aug 1, 1993·Digestive Diseases and Sciences·S OtaT Sugimoto
Jul 1, 1994·Digestive Diseases and Sciences·K YoshiuraK Okano
Oct 1, 1993·Gastroenterologia Japonica·Y TakaoS Ohshiba
Aug 30, 2001·Microscopy Research and Technique·A TeranoK Horie-Sakata
Dec 31, 1997·FEBS Letters·M TakahashiM Omata
Apr 1, 1991·Digestive Diseases and Sciences·S OtaT Sugimoto
Apr 1, 1992·Gastroenterologia Japonica·A Terano
Jan 10, 1998·European Journal of Pharmacology·E SasakiK Kobayashi
Jun 1, 1994·Prostaglandins·Y HataT Sugimoto