A monomer-trimer model supports intermittent glucagon fibril growth

Scientific Reports
Andrej KošmrljMogens H Jensen

Abstract

We investigate in vitro fibrillation kinetics of the hormone peptide glucagon at various concentrations using confocal microscopy and determine the glucagon fibril persistence length 60μm. At all concentrations we observe that periods of individual fibril growth are interrupted by periods of stasis. The growth probability is large at high and low concentrations and is reduced for intermediate glucagon concentrations. To explain this behavior we propose a simple model, where fibrils come in two forms, one built entirely from glucagon monomers and one entirely from glucagon trimers. The opposite building blocks act as fibril growth blockers, and this generic model reproduces experimental behavior well.

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S FormisanoH Edelhoch
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·P J Hagerman
Feb 3, 1995·Journal of Molecular Biology·A OrlovaE H Egelman
Sep 19, 2002·Protein Science : a Publication of the Protein Society·Daizo Hamada, Christopher M Dobson
Mar 11, 2003·American Journal of Physiology. Endocrinology and Metabolism·Guoqiang Jiang, Bei B Zhang
Feb 12, 1962·Biochimica Et Biophysica Acta·M KASAIF OOSAWA
Dec 20, 2003·Nature·Dennis J Selkoe
Dec 3, 2005·Journal of Molecular Biology·Jesper Søndergaard PedersenDaniel Erik Otzen
Jun 8, 2006·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Aug 25, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Daniel J Drucker
May 26, 2007·Biochemistry·Christian Beyschau AndersenChristian Rischel
Mar 15, 2008·Biophysical Journal·Anna Sigrid Pii SvaneNiels Chr Nielsen
Aug 22, 2009·Science·Hao Yuan Kueh, Timothy J Mitchison
Dec 17, 2009·Science·Tuomas P J KnowlesChristopher M Dobson
Feb 17, 2010·Journal of Molecular Biology·Christian Beyschau AndersenDaniel Erik Otzen
Sep 28, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Jesper Ferkinghoff-BorgMogens H Jensen
Oct 28, 2010·The FEBS Journal·Jesper S PedersenDaniel E Otzen
Sep 17, 2011·Nano Letters·Laura C Estrada, Enrico Gratton
Oct 18, 2011·Journal of the American Chemical Society·Corianne C vandenAkkerGijsje H Koenderink
May 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Samuel I A CohenTuomas P J Knowles
Nov 7, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Vladimir P Zhdanov

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
Confocal Microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
Jesper Ferkinghoff-BorgMogens H Jensen
Langmuir : the ACS Journal of Surfaces and Colloids
Francesca MacchiDaniel Erik Otzen
European Journal of Biochemistry
W B GratzerG H Beaven
© 2022 Meta ULC. All rights reserved