PMID: 6171622Jun 1, 1981Paper

A morphological study of the uptake and orthograde axoplasmic transport of horseradish peroxidase by damaged autonomic neurons in vitro

Journal of Neurocytology
F A Al-KhafajiD Mayor

Abstract

A preparation comprising the guinea-pig inferior mesenteric ganglion (IMG) and ligated hypogastric nerves was maintained in vitro in a twin-chamber apparatus. Horseradish peroxidase (HRP), added to the ganglion compartment, was taken up via coated pinocytotic vesicles into the neuronal perikarya, and subsequently accumulated in many polymorphic cytoplasmic organelles, but it did not enter the Golgi complex. After 24 h incubation, HRP was also localized in membrane-bounded organelles in the nonmyelinated axons of the hypogastric nerves in the ligated nerve compartment. HRP-labelled organelles accumulated in swollen nonmyelinated axons immediately proximal to the ligation along with other organelles known to undergo fast axoplasmic transport. It is suggested that HRP taken up by the neuronal perikarya subsequently underwent fast axoplasmic transport without passing through the Golgi complex. The organelles in which HRP was transported in an orthograde direction were very similar to those in which the enzyme is known to be transported retrogradely in the same neuronal system. The advantages of this in vitro system for studying the orthograde transport of exogenous protein are discussed.

References

Dec 1, 1976·General Pharmacology·M A Bisby
May 1, 1979·The Journal of Comparative Neurology·R D Broadwell, M W Brightman
Apr 23, 1979·Biochimica Et Biophysica Acta·D J MorréW W Franke
Apr 1, 1977·The Journal of Cell Biology·N K GonatasS Avrameas
Oct 31, 1975·Brain Research·J L BarkerR J Lasek
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky

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Citations

Jan 1, 1982·Acta Neuropathologica·J Mitchell

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