A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool Deficiency

Journal of Clinical Medicine
William T GunningNeil A Lachant

Abstract

One thousand and eighty patients, having prolonged bleeding times, frequent epistaxis, menorrhagia or easy bruising or other bleeding manifestations, and excluding those with von Willebrand's disease, were evaluated for platelet dense granule deficiency. The mean diameter of platelet dense granules was determined for all patients using image analysis. Four hundred and ninety-nine had "classic" dense (delta) granule storage pool deficiency (δ-SPD). Five hundred and eighty-one individuals (53.8%) were found to have a normal mean number of dense granules, but for some of these patients, the dense granules were smaller than for the controls. Of the patients having a normal number of dense granules, 165 (28.4%) were found to have significantly smaller granules than the platelets obtained from the control subjects. Their average granule diameter was 123.35 ± 0.86 nm, that is more than three standard deviations below the mean of the control data. Total δ-granule storage pool volumes (TDGV)/platelet were calculated using these measurements. Individuals with δ-SPD had half the number of granules (2.25 ± 0.04 DG/PL) and storage pool volume (3.88 ± 1.06 × 106 nm3) when compared to our control data (4.64 ± 0.11 DG/PL; 10.79 × 106 nm3 ± 0.4...Continue Reading

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Citations

Aug 23, 2020·Journal of Clinical Medicine·Jessica Le BlancMarie Lordkipanidzé
Aug 8, 2020·Journal of Clinical Medicine·Arnaud DupuisChristian Gachet
Oct 24, 2020·Haemophilia : the Official Journal of the World Federation of Hemophilia·Will ThomasMichael J R Desborough
Dec 24, 2021·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Ryan J ReagansWilliam T Gunning

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Methods Mentioned

BETA
X-ray
electron microscopy
transmission electron microscopy
Assay
electron
flow cytometry
exome sequencing

Software Mentioned

SigmaPlot
Image Pro
NIH Image

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