PMID: 6169526Aug 1, 1981Paper

A mouse cell line, which is unprotected by interferon against lytic virus infection, lacks ribonuclease F activity

European Journal of Biochemistry
D A EpsteinA Panet

Abstract

A mouse cell line, NIH 3T3, does not respond to some of the activities of interferon. Even after treatment with high concentrations of interferon the replication of lytic viruses, such as encephalomyocarditis virus (EMCV) and vesicular stomatitis virus (VSV) is not inhibited in these cells. In contrast, interferon treatment of these same cells results in the inhibition of Moloney murine leukemia virus (MMuLV) production. We have analyzed enzymatic pathways which are induced by interferon in these cells. After interferon treatment, the level of the (2'-5')oligoadenylate [(2'-5)An] synthetase activity and the phosphorylation of the 67000-dalton protein (P1) are enhanced in NIH 3T3 cells to approximately the same level as interferon-sensitive mouse L-cells. Moreover, NIH 3T3 and L-cells, contain approximately the same levels of enzymes which inactivate (2'-5')An. Both exogenously added (2'-5')A3 or double-stranded RNA (dsRNA) failed to inhibit protein synthesis in NIH 3T3 extracts even though they were potent inhibitors of L-cell extract-directed protein synthesis. Direct measurements of the (2'-5')An-dependent ribonuclease F (RNase F) failed to detect such activity in NIH 3T3 cells. Our results, therefore, suggest that the presen...Continue Reading

References

Oct 1, 1978·The Journal of General Virology·P VandenbusscheJ Werenne
Feb 1, 1979·European Journal of Biochemistry·A G Hovanessian, I M Kerr
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A KimchiM Revel
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A SchmidtM Revel
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·I M Kerr, R E Brown
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L A Ball, C N White
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P J FarrellP Lengyel
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E SlatteryP Lengyel
Nov 1, 1979·Nature·J N Wood, A G Hovanessian
Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Mar 14, 1968·Journal of Molecular Biology·D Baltimore
Mar 1, 1972·Journal of Virology·I M Kerr, E M Martin
Feb 1, 1974·Journal of the National Cancer Institute·I GresserD Brouty-Boyé
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M VerhaegenJ Content
Feb 1, 1980·Virology·A G Hovanessian, J N Wood

❮ Previous
Next ❯

Citations

Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G MerlinM Revel
Jan 1, 1982·Journal of Interferon Research·J C Lee, C Gauntt
Jan 1, 1982·Journal of Interferon Research·R M FriedmanL D Kohn
Jan 1, 1982·Journal of Interferon Research·D K BanerjeeR M Friedman
Dec 1, 1986·Journal of Interferon Research·Y R HsuT Arakawa
May 1, 1992·Journal of Interferon Research·J Wybier-Franqui, D Brouty-Boyé
Jan 16, 2008·Journal of Cellular and Molecular Medicine·J MagesH Adler
Jan 1, 1988·Microbiology and Immunology·A IwataY Kawade
Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·N MorinagaT Kuwata
Dec 19, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·R J MintzerA Johns
Jul 9, 2020·Viruses·Meredith C RogersJohn V Williams
Apr 30, 1998·Biochimica Et Biophysica Acta·M Tnani, B A Bayard
Mar 1, 1983·Journal of Virology·G C Sen, R E Herz

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.