A mouse chromosome-specific YAC probe collection for in situ hybridization

Genomics
F MongelardC Vourc'h

Abstract

To facilitate the identification of mouse metaphase chromosomes by fluorescence in situ hybridization (FISH), a complete collection of mouse chromosome-specific markers has been established. Yeast artificial chromosome libraries were screened by polymerase chain reaction using primers for known loci. DNAs from positive clones were then tested by FISH. One probe per chromosome was selected on the basis of high specificity (nonchimerism) and strong fluorescence.

Citations

Oct 31, 2002·The European Journal of Neuroscience·Alexandra ErvenNicholas D Allen
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·E HeardP Avner
Apr 30, 2002·Genes, Chromosomes & Cancer·Kurt A KrummelDavid I Smith
Jan 22, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M LenerT Meo
Feb 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A SabileP Avner
May 8, 1998·Methods : a Companion to Methods in Enzymology·G E Herman

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