Three major mouse knockout programs are underway worldwide, working together to mutate all protein-encoding genes in the mouse using a combination of gene trapping and gene targeting in mouse embryonic stem (ES) cells. Although the current emphasis is on production of this valuable resource, there are significant efforts to facilitate program coordination, to enhance the availability of this resource, and to plan for future efforts in mouse genetics research.
Gene targeting using a promoterless gene trap vector ("targeted trapping") is an efficient method to mutate a large fraction of genes
Integrating mouse anatomy and pathology ontologies into a phenotyping database: tools for data capture and training
Mouse large-scale phenotyping initiatives: overview of the European Mouse Disease Clinic (EUMODIC) and of the Wellcome Trust Sanger Institute Mouse Genetics Project.
The Mammalian Phenotype Ontology as a unifying standard for experimental and high-throughput phenotyping data.
Accessing data from the International Mouse Phenotyping Consortium: state of the art and future plans
Molecular characterization of mutant mouse strains generated from the EUCOMM/KOMP-CSD ES cell resource.
Derivation of rat embryonic stem cells and generation of protease-activated receptor-2 knockout rats
MASTR: a technique for mosaic mutant analysis with spatial and temporal control of recombination using conditional floxed alleles in mice
Heligmosomoides bakeri: a model for exploring the biology and genetics of resistance to chronic gastrointestinal nematode infections
A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond
Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides
Generation and analysis of partially haploid cells with Cre-mediated chromosome deletion in the lymphoid system.
Ablation of vacuole protein sorting 18 (Vps18) gene leads to neurodegeneration and impaired neuronal migration by disrupting multiple vesicle transport pathways to lysosomes
Genes and biological processes commonly disrupted in rare and heterogeneous developmental delay syndromes
PBmice: an integrated database system of piggyBac (PB) insertional mutations and their characterizations in mice
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