PMID: 6168390Jul 1, 1981Paper

A mouse homolog to the avian sarcoma virus src protein is a member of a protein kinase cascade

Cell
M SpectorE Racker

Abstract

Recent work has identified a cascade of membrane bound protein kinases in Ehrlich ascites tumor cells. These enzymes, designated PKL, PKS and PKM, are present in both Ehrlich tumor and mouse brain, but the cascade is active only in the tumor tissue. We have now purified a fourth protein kinase, PKF, that is also associated with this cascade. Protein kinase F prosphorylates PKL and is phosphorylated by PKS. The position of this kinase in the cascade is as follows, where the arrows denote phosphorylation: [Formula: see text] The phosphorylation by PKF, like phosphorylation by the other kinases, is at a tyrosine residue and causes the substrate kinase (PKL) to become active. The role of the tyrosine phosphorylation in activating these kinases is described in detail elsewhere. One result of activation of the cascade is the phosphorylation of the beta subunit of the Na+K+-ATPase, which causes inefficient Na+ pumping and is at last in part responsible for the high aerobic glycolysis of Ehrlich ascites tumor cells. By several criteria protein kinase F from Ehrlich cells is homologous to the src gene product (pp60src) from avian sarcoma viruses. Antiserum raised against PKF and sera from rabbits bearing rous sarcoma virus (RSV)-induced...Continue Reading

References

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Citations

Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ferric C FangArturo Casadevall
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J E SmartJ M Bishop
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B Z Shilo, R A Weinberg
Mar 1, 1985·European Journal of Biochemistry·F SchönholzerM E Schweingruber
Aug 19, 2007·Journal of Molecular Microbiology and Biotechnology·James B Russell
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·I B Weinstein
Aug 1, 1990·Psychological Medicine·J A BushnellP Joyce
Oct 16, 1981·Science·G B Kolata

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