A mouse line expressing Sall1-driven inducible Cre recombinase in the kidney mesenchyme

Genesis : the Journal of Genetics and Development
Shuji InoueRyuichi Nishinakamura

Abstract

Sall1 is expressed in the metanephric mesenchyme in the developing kidney, and mice deficient in Sall1 show kidney agenesis or dysgenesis. Sall1 is also expressed elsewhere, including in the limb buds, anus, heart, and central nervous system. Dominant-negative mutations of Sall1 in mice and humans lead to developmental defects in these organs. Here, we generated a mouse line expressing tamoxifen-inducible Cre recombinase (CreER(T2)) under the control of the endogenous Sall1 promoter. Upon tamoxifen treatment, these mice showed genomic recombination in the tissues where endogenous Sall1 is expressed. When CreER(T2) mice were crossed with the floxed Sall1 allele, tamoxifen administration during gestation led to a significant decrease in Sall1 expression and small kidneys at birth, suggesting that Sall1 functions were disrupted. Furthermore, Sall1 expression in the kidney was significantly reduced by neonatal tamoxifen treatment. The Sall1CreER(T2) mouse is a valuable tool for in vivo time-dependent and region-specific knockout and overexpression studies.

References

Aug 28, 1997·Biochemical and Biophysical Research Communications·R FeilP Chambon
Apr 12, 2001·BMC Developmental Biology·S SrinivasF Costantini
Aug 14, 2003·Human Molecular Genetics·Susan McLeskey KieferMichael Rauchman
Jun 3, 2004·Mechanisms of Development·Minoru TakasatoRyuichi Nishinakamura
Jan 18, 2008·Kidney International·R Nishinakamura
Jan 27, 2009·Development·Yasuhiko KawakamiJuan Carlos Izpisua Belmonte

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Citations

Feb 22, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Ryuichi NishinakamuraSayoko Fujimura
Nov 11, 2010·The Journal of Biological Chemistry·Efthimia KarantzaliAndroniki Kretsovali
Feb 16, 2016·Journal of Molecular and Cellular Cardiology·Yuika MoritaJun K Takeuchi
Nov 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kentaro SuzukiGen Yamada
Dec 29, 2013·Developmental Biology·Aki MurashimaGen Yamada
Sep 30, 2017·International Journal of Molecular Sciences·Tobias Koeniger, Stefanie Kuerten
Apr 20, 2014·Journal of the American Society of Nephrology : JASN·Shoichiro KandaRyuichi Nishinakamura
Nov 8, 2017·Journal of the American Society of Nephrology : JASN·Sepideh Sheybani-DelouiNorman D Rosenblum
Nov 26, 2019·European Journal of Immunology·Louise Chappell-MaorSteffen Jung
Dec 14, 2018·The Journal of Experimental Medicine·Kevin A Guttenplan, Shane A Liddelow
Dec 22, 2019·Journal of the American Society of Nephrology : JASN·Shoichiro KandaMotoshi Hattori
Oct 30, 2020·Frontiers in Immunology·Tomás A MartinsGregor Hutter
Feb 2, 2021·Autophagy·Keertana Srimat KandadaiJan D Lünemann
Jul 14, 2021·Congenital Anomalies·Mellissa C AlcantaraGen Yamada

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