A mouse model for chronic intermittent electronic cigarette exposure exhibits nicotine pharmacokinetics resembling human vapers

Journal of Neuroscience Methods
Xuesi M ShaoTheodore C Friedman

Abstract

Electronic cigarettes (E-cig) use is increasing rapidly, particularly among youths. Animal models for E-cig exposure with pharmacokinetics resembling human E-cig users are lacking. We developed an E-cig aerosol exposure system for rodents and a chronic intermittent delivery method that simulates E-cig users who vape episodically during wakefulness and abstain during sleep. Mice were exposed to E-cig in a programmed schedule at very low, low, medium, or high doses defined by duration of each puff, number of puffs per delivery episode and frequency of episodes in the dark phase of a 12/12-h circadian cycle for 9 consecutive days. The plasma nicotine/cotinine levels and their time courses were determined using LC/MS-MS. We assessed the body weight, food intake and locomotor activity of Apolipoprotein E null (ApoE-/-) mice exposed to chronic intermittent E-cig aerosol. Plasma nicotine and cotinine levels were positively correlated with exposure doses. Nicotine and cotinine levels showed a circadian variation as they increased with time up to the maximum nicotine level of 21.8 ± 7.1 ng/mL during the daily intermittent E-cig exposure in the 12-h dark phase and then declined during the light phase when there was no E-cig delivery. Chr...Continue Reading

Citations

Jan 23, 2020·International Journal of Molecular Sciences·Anna Merecz-SadowskaRadoslaw Zajdel
Jun 28, 2020·Neuropharmacology·Asti JacksonSuchitra Krishnan-Sarin
Mar 24, 2021·Pharmacology & Therapeutics·Emily BonnerRobyn L Tanguay
Sep 16, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Andrew WalayatDaliao Xiao

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