A mouse model of cavernous nerve injury-induced erectile dysfunction: functional and morphological characterization of the corpus cavernosum

The Journal of Sexual Medicine
Hai-Rong JinJun-Kyu Suh

Abstract

With the advent of genetically engineered mice, it seems important to develop a mouse model of cavernous nerve injury (CNI). To establish a mouse model of CNI induced either by nerve crushing or by neurectomy and to evaluate time-dependent derangements in penile hemodynamics in vivo and subsequent histologic alterations in the cavernous tissue. Twelve-week-old C57BL/6J mice were divided into 4 groups (N=36 per group): control, sham operation, bilateral cavernous nerve crush, and bilateral cavernous neurectomy group. Three days and 1, 2, 4, 8, and 12 weeks after CNI, erectile function was measured by electrical stimulation of the cavernous nerve. The penis was then harvested and TUNEL was performed. Immunohistochemical analysis was performed assaying for caspase-3, transforming growth factor-β1 (TGF-β1), phospho-Smad2, PECAM-1, factor VIII, and smooth muscle α-actin. The numbers of apoptotic cells and phospho-Smad2-immunopositive cells in endothelial cells or smooth muscle cells were counted. Erectile function was significantly less in the cavernous nerve crushing and neurectomy groups than in the control or sham group. This difference was observed at the earliest time point assayed (day 3) and persisted up to 4 weeks after nerv...Continue Reading

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Citations

Oct 5, 2011·The Journal of Sexual Medicine·Tao LiuZhong-Cheng Xin
Mar 19, 2014·The Journal of Sexual Medicine·Johanna L HannanTrinity J Bivalacqua
Apr 16, 2011·The Journal of Sexual Medicine·Shinichi YamashitaTaiji Tsukamoto
Dec 20, 2011·The Journal of Urology·Sena F SezenArthur L Burnett
May 31, 2011·The Urologic Clinics of North America·Clarisse Mazzola, John P Mulhall
Apr 26, 2016·International Journal of Urology : Official Journal of the Japanese Urological Association·Mi-Hye KwonJun-Kyu Suh
Sep 23, 2016·International Journal of Impotence Research·O KwonS W Kim

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