A mouse model of cytogenetic analysis to evaluate caesium137 radiation dose exposure and contamination level in lymphocytes

Radiation and Environmental Biophysics
Sandrine Roch-LefèvreJoan Francesc Barquinero

Abstract

In case of external overexposure to ionizing radiation, an estimation of its genotoxic effects on exposed individuals can be made retrospectively by the measurement of radiation-induced chromosome aberrations on circulating lymphocytes. Compared with external irradiation, intakes of radionuclides may, however, lead to specific features influencing dose distribution at the scale of body, of tissue or even of cell. Therefore, in case of internal contamination by radionuclides, experimental studies, particularly using animal models, are required to better understand mechanisms of their genotoxic effects and to better estimate the absorbed dose. The present study was designed to evaluate a cytogenetic method in mouse peripheral blood lymphocytes that would allow determination of yields and complexities of chromosome aberrations after low-dose rate exposure to (137)Cs delivered in vitro either by irradiation or by contamination. By using M-FISH analysis, we compared the low-dose rate responses observed in mouse to the high-dose rate responses observed both in mouse and in human. Promising similarities between the two species in the relative biological effect evaluation show that our cytogenetic model established in mouse might be us...Continue Reading

References

Jan 1, 1987·Environmental and Molecular Mutagenesis·G L Erexson, A D Kligerman
Dec 6, 2002·Nature·UNKNOWN Mouse Genome Sequencing ConsortiumEric S Lander
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Aug 24, 2010·Radiation Research·Volodymyr A VinnikovKai Rothkamm
Dec 25, 2010·Radiation Protection Dosimetry·E A AinsburyK Rothkamm

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Citations

Feb 26, 2019·Radiation Research·Monica Pujol-CanadellLubomir Smilenov
Jul 7, 2018·Dose-response : a Publication of International Hormesis Society·Noriko Shimura, Shuji Kojima
May 21, 2017·Radiation and Environmental Biophysics·Igor K KhvostunovShunichi Yamashita

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