A mouse model to study infection against porcine circovirus type 2: viral distribution and lesions in mouse.

Virology Journal
Jun LiHongbin Ai

Abstract

Little information is known about viral distribution and transmission of porcine circovirus type 2 (PCV2) in species other than swine. It is still a debated topic whether the PCV2 could be infected and caused clinical lesions. Our study is aimed to estimate the susceptibility of Kunming mouse to PCV2. Forty-eight, 6-week-old Kunming mice were randomly divided into four groups. Group A (C1-C12) was inoculated with PK-15 cell culture as a control group. Group B (sPCV1-12) was inoculated orally and intramuscularly with PCV2 (10(6.2)TCID50/ml). Group C (mPCV1-12) was inoculated orally and intramuscularly with PCV2 (10(6.2)TCID50/ml) and a booster inoculation at days 14 and 28 after the first inoculation. Group D (MixPCV1-12) was unvaccinated but released into Group C. Each group was sacrificed at 7, 14, 28, and 42 days post-inoculation, respectively. Necropsy was checked on every mouse. Sera samples were collected for the test of PCV2 specific antibody. Tissues were collected for histopathology study and polymerase chain reaction (PCR). The results showed that viral replication, seroconversion, and microscopic lesions were found in inoculated mice. Continuous existence of PCV2 viruses in lymph nodes have been confirmed by PCR, whic...Continue Reading

References

Jan 30, 1999·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J EllisF McNeilly
Jun 12, 2002·Veterinary Research·Josefina QuintanaMariano Domingo
Nov 2, 2005·BMC Veterinary Research·Matti KiupelSuresh K Mittal

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Citations

Jan 29, 2013·The British Journal of Nutrition·Wenkai RenYulong Yin
Feb 11, 2016·International Journal of Environmental Research and Public Health·Linfu ZhouGang Zhao
Dec 19, 2015·Research in Veterinary Science·Alessandra M M G de CastroLeonardo J Richtzenhain
Mar 27, 2016·Virus Genes·Linzhu RenHongsheng Ouyang
Jun 5, 2015·Clinical and Experimental Pharmacology & Physiology·Xin YangLinzhu Ren
May 11, 2013·Acta Veterinaria Hungarica·Zhi Bang DengLi Qun Xue
Nov 13, 2012·Research in Veterinary Science·Albanno Leonard Braz Campos PinheiroAbelardo Silva Júnior
Mar 21, 2019·Animal Models and Experimental Medicine·Ting OuyangLin-Zhu Ren
Oct 17, 2019·Frontiers in Veterinary Science·Shao-Lun ZhaiYun Xi
Apr 12, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chen Hai-LanHu Ting-Jun

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Methods Mentioned

BETA
PCR
ELISA
electrophoresis
Enzyme-linked immunosorbent assay

Software Mentioned

SPSS13

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