Abstract
Failure of trabeculectomy is attributed to an exaggerated wound healing response at the episcleral level. We have developed a simple technique in mice that creates corneoscleral wounds and used it to study the role of growth factors in wound healing associated with loss of fistular patency. In addition, the effect of suramin on the wound healing response has been investigated. Using black C57B1/6 mice, we created a fistula from the subconjunctival space to the anterior chamber by external penetration with a 25-gauge needle through the bulbur conjunctiva. Eyes were examined by light microscopy at different times following surgery and evaluated for the presence of growth factors in the sclerosing wound by immunohistochemistry. In an additional group, suramin (1.0 mg/ml) was applied topically five times. The limbal/scleral wounds closed rapidly. Granulation tissue consisting mostly of fibroblasts developed within the first 2 days, and the collagen component increased over time. By 4 weeks, the wound was completely healed. We found that within 1 day after surgery GF beta 2 reached high intensity. TGF beta 1 and PDGF A reached maximal intensity by day 2 and remained elevated for about a week, PDGF B was present at moderate intensity...Continue Reading
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