A multi-analyte biosensor for the simultaneous label-free detection of pathogens and biomarkers in point-of-need animal testing

Analytical and Bioanalytical Chemistry
Melanie EwaldGünter Gauglitz

Abstract

For the first time, a multi-analyte biosensor platform has been developed using the label-free 1-lambda-reflectometry technique. This platform is the first, which does not use imaging techniques, but is able to perform multi-analyte measurements. It is designed to be portable and cost-effective and therefore allows for point-of-need testing or on-site field-testing with possible applications in diagnostics. This work highlights the application possibilities of this platform in the field of animal testing, but is also relevant and transferable to human diagnostics. The performance of the platform has been evaluated using relevant reference systems like biomarker (C-reactive protein) and serology (anti-Salmonella antibodies) as well as a panel of real samples (animal sera). The comparison of the working range and limit of detection shows no loss of performance transferring the separate assays to the multi-analyte setup. Moreover, the new multi-analyte platform allows for discrimination between sera of animals infected with different Salmonella subtypes.

References

Jul 5, 2005·Analytical and Bioanalytical Chemistry·Florian PröllGünter Gauglitz
Jul 12, 2005·Journal of Theoretical Biology·Geoff Wild, Peter D Taylor
Jul 7, 2007·Journal of Chromatography. a·Guenther ProllGuenter Gauglitz
May 4, 2011·Analytical and Bioanalytical Chemistry·L Leidner, G Gauglitz
Dec 20, 2012·Environmental Science and Pollution Research International·Sara E McNameeKatrina Campbell
May 21, 2013·Analytical and Bioanalytical Chemistry·Melanie EwaldGünther Proll
Nov 28, 2013·Analytical and Bioanalytical Chemistry·Oliver BleherGünther Proll
May 13, 2014·Analytical and Bioanalytical Chemistry·Peter FechnerJulia Widmaier

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Citations

Feb 3, 2016·Biotechnology Advances·Brian M CumminsGlenn M Walker
Oct 12, 2017·Analytical and Bioanalytical Chemistry·Günter Gauglitz
Jul 26, 2018·Sensors·Witold NawrotKarol Malecha

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