A Multi-Camera System for Bioluminescence Tomography in Preclinical Oncology Research

Diagnostics
Matthew A LewisRalph P Mason

Abstract

Bioluminescent imaging (BLI) of cells expressing luciferase is a valuable noninvasive technique for investigating molecular events and tumor dynamics in the living animal. Current usage is often limited to planar imaging, but tomographic imaging can enhance the usefulness of this technique in quantitative biomedical studies by allowing accurate determination of tumor size and attribution of the emitted light to a specific organ or tissue. Bioluminescence tomography based on a single camera with source rotation or mirrors to provide additional views has previously been reported. We report here in vivo studies using a novel approach with multiple rotating cameras that, when combined with image reconstruction software, provides the desired representation of point source metastases and other small lesions. Comparison with MRI validated the ability to detect lung tumor colonization in mouse lung.

References

Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·B J TrombergJ Butler
Dec 1, 2001·Journal of Biomedical Optics·B W RiceM B Nelson
May 30, 2003·Medical Physics·Edward E GravesVasilis Ntziachristos
Apr 17, 2004·Cancer Research·Joel R GarbowMing You
Jul 6, 2004·The Journal of Surgical Research·Shiva Sarraf-YazdiBryan M Clary
Sep 21, 2004·Medical Physics·Ge WangMing Jiang
Sep 24, 2005·Physics in Medicine and Biology·George AlexandrakisArion F Chatziioannou
Nov 25, 2005·Physics in Medicine and Biology·Abhijit J ChaudhariRichard M Leahy
Feb 17, 2006·Optics Letters·Hamid DehghaniMichael S Patterson
Feb 21, 2006·Medical Physics·Nikolai V SlavinePeter P Antich
Sep 13, 2006·Journal of Biomedical Optics·E Duco JansenJohn Virostko
Apr 21, 2007·Applied Optics·Vadim Y Soloviev
Apr 28, 2007·Annual Review of Biomedical Engineering·Victor VillalobosDavid Piwnica-Worms
May 5, 2007·Journal of Biomedical Optics·Chaincy KuoBrad W Rice
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Ge WangYue Wang
Feb 15, 2008·International Journal of Biomedical Imaging·Zhun XuJing Bai
Feb 22, 2008·Applied Optics·Mehmet Burcin Unlu, Gultekin Gulsen
Apr 4, 2008·Nature·Ralph Weissleder, Mikael J Pittet
Jun 11, 2008·Optics Express·Ge WangPurnima Dubey
Jul 2, 2008·Physics in Medicine and Biology·Sangtae AhnRichard M Leahy
Nov 22, 2008·Journal of Biomedical Optics·Emilie RoncaliBertrand Tavitian
Dec 17, 2008·Medical Physics·Hamid DehghaniBrian W Pogue
Aug 23, 2004·Optics Express·Xuejun GuHuabei Jiang
Sep 5, 2005·Optics Express·Wenxiang CongAlexander Cong
Aug 21, 2006·Optics Express·Ge WangGuo Wei Wei
Aug 18, 2009·Methods in Molecular Biology·Angelina ConteroRalph P Mason
Dec 8, 2009·Current Opinion in Chemical Biology·Jennifer A Prescher, Christopher H Contag
Dec 8, 2009·The Journal of Pathology·Karen O'NeillAnnette T Byrne
Aug 4, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Changqing LiSimon R Cherry
May 9, 2012·The Review of Scientific Instruments·Han YanGultekin Gulsen

❮ Previous
Next ❯

Citations

Nov 19, 2019·International Journal of Radiation Oncology, Biology, Physics·Zijian DengKen Kang-Hsin Wang
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li LiuRalph P Mason
Nov 16, 2021·ACS Chemical Biology·Shirley LiuJohn A Ronald

❮ Previous
Next ❯

Methods Mentioned

BETA
light scattering
imaging techniques

Software Mentioned

BLT
COMSOL Multiphysics
Linux
GalactonPlus
mBLT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.