Mar 31, 2020

A taxonomy of transcriptomic cell types across the isocortex and hippocampal formation

BioRxiv : the Preprint Server for Biology
Yasin ?enbabao?luHongkui Zeng

Abstract

The isocortex and hippocampal formation are two major structures in the mammalian brain that play critical roles in perception, cognition, emotion and learning. Both structures contain multiple regions, for many of which the cellular composition is still poorly understood. In this study, we used two complementary single-cell RNA-sequencing approaches, SMART-Seq and 10x, to profile ~1.2 million cells covering all regions in the adult mouse isocortex and hippocampal formation, and derived a cell type taxonomy comprising 379 transcriptomic types. The completeness of coverage enabled us to define gene expression variations across the entire spatial landscape without significant gaps. We found that cell types are organized in a hierarchical manner and exhibit varying degrees of discrete or continuous relatedness with each other. Such molecular relationships correlate strongly with the spatial distribution patterns of the cell types, which can be region-specific, or shared across multiple regions, or part of one or more gradients along with other cell types. Glutamatergic neuron types have much greater diversity than GABAergic neuron types, both molecularly and spatially, and they define regional identities as well as inter-region re...Continue Reading

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Mentioned in this Paper

Study
Biochemical Pathway
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The Cancer Genome Atlas
Cellular Process
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Genome
Mitogen-Activated Protein Kinases
Post-Translational Protein Processing
Regulation of Biological Process

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